| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,573,157 | A→T | I148N (ATC→AAC) | yddA ← | ABC transporter ATP‑binding protein/permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,573,157 | 0 | A | T | 93.3% | 47.7 / ‑3.9 | 16 | I148N (ATC→AAC) | yddA | ABC transporter ATP‑binding protein/permease |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base T (5/9); minor base C (0/1); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
AATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGT > NZ_CP009273/1573087‑1573238 | aaTAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTc < 1:42969/90‑1 (MQ=255) aaaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACg > 2:553793/1‑90 (MQ=255) aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt < 1:89894/90‑1 (MQ=255) aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt < 2:576017/90‑1 (MQ=255) tGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGt < 2:423707/90‑1 (MQ=255) gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTc < 1:362571/58‑1 (MQ=255) gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc > 1:134934/1‑90 (MQ=255) aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa > 1:222703/1‑90 (MQ=255) aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa > 1:475807/1‑90 (MQ=255) aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa > 2:340434/1‑90 (MQ=255) gCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGCTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATc < 2:11680/90‑1 (MQ=255) gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc < 2:200631/90‑1 (MQ=255) gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc < 2:371129/90‑1 (MQ=255) aCTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGt < 2:519510/90‑1 (MQ=255) gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGaa > 2:174451/1‑90 (MQ=255) ttttGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAagtagt < 2:428910/90‑1 (MQ=255) | AATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGT > NZ_CP009273/1573087‑1573238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |