| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 120,483 | A→C | K327T (AAA→ACA) | aceE → | pyruvate dehydrogenase (acetyl‑transferring), homodimeric type |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 120,483 | 0 | A | C | 87.5% | 40.1 / ‑0.1 | 16 | K327T (AAA→ACA) | aceE | pyruvate dehydrogenase (acetyl‑transferring), homodimeric type |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (9/5); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01 | |||||||||||
GGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGC > NZ_CP009273/120400‑120569 | ggATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGaa > 1:278339/1‑90 (MQ=255) aCTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATgg < 2:199092/90‑1 (MQ=255) gctgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGc > 1:230454/1‑90 (MQ=255) gctgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTAACATCGAAAGAGGGTGc > 1:139082/1‑90 (MQ=255) tgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGt < 1:7127/90‑1 (MQ=255) cGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTAc < 1:554091/90‑1 (MQ=255) aCCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGAGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGaa > 2:410097/1‑90 (MQ=255) ggTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACActtc > 1:246481/1‑90 (MQ=255) ggTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACActtc > 1:330107/1‑90 (MQ=255) tAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACActtctt < 2:246481/90‑1 (MQ=255) gATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGGGCGTACGTTCGTGAACACTTCTTCGGTaa > 2:589311/1‑90 (MQ=255) tCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATgg > 1:205265/1‑58 (MQ=255) tCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATgg < 2:205265/58‑1 (MQ=255) aGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATc > 1:276588/1‑90 (MQ=255) gCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAg > 1:121210/1‑90 (MQ=255) tCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGc < 1:410097/90‑1 (MQ=255) | GGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGC > NZ_CP009273/120400‑120569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |