Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,576,388	A→G	S132P (TCC→CCC)	ydeN ←	sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,576,388	0	A	G	100.0%	38.7 / NA	14	S132P (TCC→CCC)	ydeN	sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/4);  total (10/4)

TACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAG  >  NZ_CP009273/1576304‑1576471                                                                                     |                                                                                    tACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAacac                                                                                <  2:162137/90‑1 (MQ=255)     aTCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCgtg                                                                            >  1:101905/1‑90 (MQ=255)      tCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCgtgt                                                                           <  2:168885/90‑1 (MQ=255)          gAGCGTCGGGATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGCGCca                                                                       >  1:268518/1‑90 (MQ=255)          gAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCca                                                                       >  2:429202/1‑90 (MQ=255)                 ggTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGcc                                                                <  2:90844/90‑1 (MQ=255)                            aGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAAc                                                     >  1:382386/1‑90 (MQ=255)                            aGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAAc                                                     >  2:195250/1‑90 (MQ=255)                               cacCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTa                                                  >  2:410389/1‑90 (MQ=255)                                         ggcgggAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATc                                        >  1:269900/1‑90 (MQ=255)                                                        ggTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAg                         >  2:173908/1‑90 (MQ=255)                                                            attattGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTc                     <  1:191945/90‑1 (MQ=255)                                                                       gcgGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGAttt          >  2:53117/1‑90 (MQ=255)                                                                               ggCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAg  >  1:122052/1‑90 (MQ=255)                                                                                     |                                                                                    TACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAG  >  NZ_CP009273/1576304‑1576471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑