| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,579,109 | A→C | G545G (GGT→GGG) | ydeP ← | acid resistance putative oxidoreductase YdeP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,579,109 | 0 | A | C | 96.3% | 82.9 / ‑4.6 | 27 | G545G (GGT→GGG) | ydeP | acid resistance putative oxidoreductase YdeP |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (15/11); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGCA > NZ_CP009273/1579023‑1579194 | accaGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcg > 1:309373/1‑90 (MQ=255) caGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc < 1:296977/90‑1 (MQ=255) cAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAac < 1:71781/90‑1 (MQ=255) aGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAaca > 1:357973/1‑90 (MQ=255) gggTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATg < 2:472562/90‑1 (MQ=255) gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca > 1:53923/1‑90 (MQ=255) gctgcCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatcatc < 1:263791/90‑1 (MQ=255) gctgcCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatcatc > 1:287732/1‑90 (MQ=255) tgcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa > 2:285249/1‑90 (MQ=255) tgcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa < 2:309373/90‑1 (MQ=255) gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGGTTTAACACGCCACGCGAGGCATGAATCATCGACATTg > 1:465788/1‑90 (MQ=255) cgcgATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGa > 2:114920/1‑90 (MQ=255) gcgATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGaa < 1:179907/90‑1 (MQ=255) cccGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCa < 1:168242/90‑1 (MQ=255) cgcgACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAAc < 2:446923/90‑1 (MQ=255) aCTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc < 1:285249/90‑1 (MQ=255) ctGATTTCAGCATTACCCCGGCGGGTTTTAAcacgccacgc < 1:389298/41‑1 (MQ=255) ctGATTTCAGCATTACCCCGGCGGGTTTTAAcacgccacgc > 2:389298/1‑41 (MQ=255) tGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCa < 1:281119/70‑1 (MQ=255) tGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCa > 2:281119/1‑70 (MQ=255) tGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCAcc > 2:525490/1‑90 (MQ=255) tGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCAcc > 1:475490/1‑90 (MQ=255) ttCAGCATTACCCCGGCGGGTTTTAACCCGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGttt < 1:461692/90‑1 (MQ=255) aGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGtttttt > 2:123411/1‑90 (MQ=255) aGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGtttttt > 2:464464/1‑90 (MQ=255) aGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGtttttt > 1:445699/1‑90 (MQ=255) ttACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGCa > 2:176225/1‑90 (MQ=255) | ACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGCA > NZ_CP009273/1579023‑1579194 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |