| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 262,051 | 0 | T | G | 54.5% | 4.6 / 12.4 | 11 | T72P (ACC→CCC) | BW25113_RS25425 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGT‑CAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCAC > NZ_CP009273/261994‑262132 | gCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGc < 2:265437/71‑1 (MQ=255)gCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACTGc > 1:265437/1‑71 (MQ=255) atCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGT‑c < 1:313999/90‑1 (MQ=255) tagtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTCCAAGGGGGGGCCGGGGAAGTAGT‑CAAACcgtggc > 2:212052/1‑88 (MQ=21) aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGGAAAAACCCCAAGGGGGGGCCGGGGAAAAAGT‑CAAACCGTGACCcttctt > 2:308714/1‑90 (MQ=255) cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTTCAAGGGGGGGGCGGGTAAAAAGA‑CAAACCGTGACCCttttttc > 1:427988/1‑88 (MQ=255) cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAAGGAAAAACCGCAAGGGGGGGCCCGGAAAGGAGTCCAACCTG‑GGCCCttcttct > 2:382551/1‑89 (MQ=255) cATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAGGGGGGGCCGGGTAAATAGT‑CAACCTGTGGCCCTTTTTTGCTGa > 1:236820/1‑90 (MQ=255) gCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGT‑CAACCTGTGACCATTCTTCGCTGACATCATCCtg < 1:212052/90‑1 (MQ=255) cATCGGGCGACTGGCGGGGACGTAAAAACCGCAAAGGGGGGCCGGGGAAAAAGG‑CAACCTGTGGCCCTTTTTCGCTGGCATCCTCCtggg > 2:120873/1‑89 (MQ=255) cTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGT‑CAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACcac < 1:382068/90‑1 (MQ=255) | GCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGT‑CAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCAC > NZ_CP009273/261994‑262132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |