| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 700,026 | 0 | T | G | 53.3% | 12.7 / 16.2 | 16 | G209G (GGT→GGG) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTAC‑GGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATG > NZ_CP009273/699949‑700110 | ctgATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTAC‑GGTTTTCCAc < 1:241472/90‑1 (MQ=255) gATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGAC‑GGGTTTCCACgg > 1:167087/1‑90 (MQ=255) cccAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGAC‑GGGTTTTCACGGGGa > 2:177946/1‑90 (MQ=255) ggTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTAC‑GGTTTTCCACGGTGACATTAAc > 1:417876/1‑90 (MQ=255) gTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTAC‑GGTTTTCCACGGTGACATTAAcc < 2:146750/90‑1 (MQ=255) gTCCGCATCAGGTACTGAACACCGTCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTAC‑GGTTTTCCACGGTGACATTAAcc < 2:475510/90‑1 (MQ=255) aGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGAC‑GGGGTTTCACGGGGGCAATAAACCCTTCTATg > 1:105702/1‑90 (MQ=255) ggTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTAC‑GGTTTTCCACGGTGACATTAACCGCTTCTATGc < 1:87664/90‑1 (MQ=255) cTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCCCCGG‑GACATTAACCGCTTCTATTCCggg > 2:510865/1‑89 (MQ=255) ccATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGAC‑GGGTTTTCCCGGGGGCATTATCCGCTTCTATGCCGGGGACGGcc > 2:95395/1‑89 (MQ=255) gTTCCAGATTGGTGAATTCACCAACGCGGCGGGGAC‑GGGTTTCCACGGTGACATTAACCGCTTCTATGCCGGGGACGGCACCgggggggg > 1:280270/1‑88 (MQ=255) ggTGAATTCACCAACGCGGCGGGTAC‑GGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTcc < 1:177946/90‑1 (MQ=255) gAATTCACCAACGCGGCGGGGAC‑GGGTTTCCCCGGGGGCATTAACCGCTTCTATGCCGGGGGCGGCCCCGCGGGGGTGTTCATGTCCGGc > 2:609293/1‑90 (MQ=255) cAACGCGGCGGGTAC‑GGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTccc < 1:70216/90‑1 (MQ=255) gcggcgGGGAC‑GGGTTTTCCCGGTGACATTAACCGCTTCTATGCCGGGGACGGCCCCGCGGGGGTGTTCATGTCCGGCTTCTTCCCGATc > 2:55909/1‑90 (MQ=255) gcggGCAC‑GGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCatg > 1:626043/1‑90 (MQ=255) | CTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTAC‑GGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATG > NZ_CP009273/699949‑700110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |