| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 954,525 | 0 | T | G | 56.2% | 13.7 / 17.8 | 16 | G86G (GGT→GGG) | aroA | 3‑phosphoshikimate 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGC > NZ_CP009273/954458‑954610 | ataCGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCGTTACACGCGGAAGGGGGCCTGGGGATGTTCCTCggga > 2:225810/1‑88 (MQ=255)ataCGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTACACGCAGAAGGGGCCCTGGAGTTGTTTCTCggga > 2:12996/1‑88 (MQ=255) cTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGGCCATTAAACGCAGAAGGTGGCCTGGAGGTGGTTCTCGGGAACGcc > 1:302883/1‑90 (MQ=255) ttCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTTCACGCGGAAGGGGCCCTGGGGGTGTTCCTCGGGAACGCCgg > 2:317072/1‑90 (MQ=255) ttCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTACACGCAGAAGGGGCCCTGGGGTTGTTCCTCGGTAACGCCgg > 1:339950/1‑90 (MQ=255) ttGCGAAATTATCGGTAACGGCGGTCCCTTACACCCAGAAAGGGCCCTGGAGGTTTTCCCCCGGAACGCCGGAACCGCAAAGCGTCCGCt > 2:446759/1‑90 (MQ=255) ttGCGAAATTATCGGTAACGGCGGTCCATTACACGCAGAAGGGGGCCTGGGGGTGTTTCTCGGGAACGCCGGGACGGCAAAGCGGCCGCt > 2:329578/1‑90 (MQ=255) aTTATCGGTAACGGCGGTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCa < 2:288654/90‑1 (MQ=255) ttATCGGTAACGGCGGTCCCTTGCACGCCGCAGGGGCCCCGGAGTTGTTCCTCGGTAACGCCGGCACGGCAAAGCGTCCGCTGGCGGCAg > 2:605041/1‑90 (MQ=255) aCGGCGGTCCATTACACGCAGAAGGGGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAAAGCGGCCGCTGGCGGCAGGTCTTTGtt > 2:38713/1‑89 (MQ=255) gcggTCCATTACACGCAGAAGGGGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGGGGCAGCTCTTTGTCTgg > 2:71151/1‑90 (MQ=255) gTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTa < 1:12996/90‑1 (MQ=255) gTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTa < 1:299271/90‑1 (MQ=255) ttACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAAt < 1:192977/90‑1 (MQ=255) cAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATtg > 1:74269/1‑90 (MQ=255) aaGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGc < 2:418652/90‑1 (MQ=255) | ATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGC > NZ_CP009273/954458‑954610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |