Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,175,9460CG54.5% 3.6 / 10.2 11A13G (GCA→GGA) zupTzinc transporter ZupT
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGC  >  NZ_CP009273/3175857‑3176030
                                                                                         |                                                                                    
atcaGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGc                                                                                      >  1:353947/1‑90 (MQ=255)
             aCGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTAtt                                                                         <  2:353947/90‑1 (MQ=255)
                      cTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTGGCGCGttt                                                                >  1:170266/1‑90 (MQ=255)
                                          ggAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCaaaa                                            <  2:152807/90‑1 (MQ=255)
                                           gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGGTTTTTGGGGCGTTTTTCCGCCGTCTCCGGCaaaaa                                           >  2:8724/1‑90 (MQ=255)
                                             gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGGGCGTTTTTCCGCGCTCTCCGGCaaaaaac                                         >  2:555603/1‑88 (MQ=255)
                                                               tctcATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGCGCGGTTTTCTGCGTTCTCGGGGGAAAAACCCCCCACAGCTTTCTGGc                       >  2:452824/1‑90 (MQ=255)
                                                               tctcATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTGCGCGTTTTTCCGCGTTCTCGGGCAAAAAAACTCGAACCGCTTTCTGGc                       >  1:53621/1‑90 (MQ=255)
                                                                        gACCATACTGGCGGGGGGAGCCCCGTTTTTTTGGGCGTTTTTCCGCGTTCTCCGGCGAAAAACCCCCAACCGCTTTCTGGGGGTTTCggt              >  2:315681/1‑88 (MQ=255)
                                                                         aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa             <  1:383997/90‑1 (MQ=255)
                                                                         aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa             <  2:358556/90‑1 (MQ=255)
                                                                                    gggggCAGCCACGTTTTTTTGCGCGGTTTTCGGCGTTCTCGGGCAAAAAACCCCGAACCCCTTACTGGCGTTTTTGCTGGGGTTTgcggc  >  2:27607/1‑90 (MQ=255)
                                                                                         |                                                                                    
ATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGC  >  NZ_CP009273/3175857‑3176030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑