| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,774,215 | 0 | T | G | 57.9% | 17.4 / 20.8 | 19 | G343G (GGT→GGG) | lldD | quinone‑dependent L‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (11/0); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGTCGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCA > NZ_CP009273/3774126‑3774301 | gATGTCGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGgt < 2:278140/90‑1 (MQ=255) cGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAAc < 1:172880/90‑1 (MQ=255) cGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAAc < 1:610818/90‑1 (MQ=255) ttGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGGTAACCTGCTaa > 2:311613/1‑90 (MQ=255) gcgcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAAc < 2:441531/81‑1 (MQ=255) gcgcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGGTAAACTTCTCAAt > 2:429128/1‑90 (MQ=255) gcgcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAAc > 1:441531/1‑81 (MQ=255) gcgcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAAt > 2:106541/1‑90 (MQ=255) cTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTg < 1:461369/90‑1 (MQ=255) acacCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAag < 2:315762/90‑1 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGAt > 2:39473/1‑90 (MQ=255) gctgGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCCGCTAAAACTGATCGAAAAAGAGATGAAAGt > 2:510737/1‑90 (MQ=255) gctgGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCTAACCTGCTAAAACTGGTCGAAAAAGAGAGGAAAgg > 2:399797/1‑89 (MQ=255) tgGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAAAGATGAAAGTgg > 1:67269/1‑90 (MQ=255) gTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGGGGCgg > 2:15106/1‑89 (MQ=255) gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGGGGCGATgg > 2:354754/1‑89 (MQ=255) gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGGGATGa > 2:110143/1‑90 (MQ=255) cgcTGGCAACAGCGGGCCAGGCGGGGGTAGCCAACCTGCTAAAACTCCACGAAAAAAAGGGGAAAAAGGCCATGTCGCTGCCTGGggcgg > 1:458701/1‑89 (MQ=255) cGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGc < 2:451939/90‑1 (MQ=255) gggTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCa < 1:15106/90‑1 (MQ=255) | GATGTCGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCA > NZ_CP009273/3774126‑3774301 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |