| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,977,450 | 0 | T | C | 58.8% | 11.1 / 18.6 | 17 | F378S (TTC→TCC) | aslB | anaerobic sulfatase maturase |
| Reads supporting (aligned to +/- strand): ref base T (6/1); new base C (0/10); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGGGAGGCTGCCCGAAACACCGCTTCATGCTCGATGCCAGCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTG > NZ_CP009273/3977362‑3977535 | tGGGGAGGCTGCCCGAAACACCGCTTCATGCTCGATGCCAGCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTc < 2:389457/90‑1 (MQ=255) cccGAACCCCCGCTTCATGCTCGATGCCAGCGGCAACCCGGGACTAAATTTTTTGTGGGCCGGGTATCAGCGTTTTTCCCGCCATCTAcc < 2:130968/90‑1 (MQ=255) aaCCCCGCTTCATGCCCGATGCCCCCGCAAACCCGGGACTAAATTTTTTGTGTGCCGGGTATCAGCTTTATTCCCCCCATCTACCGCCat < 2:37744/90‑1 (MQ=255) cGATGCCAGCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGc > 2:421802/1‑90 (MQ=255) gCGGCAACCGGGGACTGATTTTTTTGTGGGCGGGGTATCGGTTTTTTTCCCCCCATCTCCCGCCATATCTTAAAGCAATGGCTGATTTGc < 2:377101/90‑1 (MQ=255) gCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGc > 1:395036/1‑90 (MQ=255) gggACTAAATTATTTGTGTGCCGGGTATCAGCTTTTTTCCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACgg < 1:567535/90‑1 (MQ=255) tttGTGTGCCGGTTATCAGCTTTTTTCCCCCCATCTCCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAg < 2:294335/90‑1 (MQ=255) ttGTGTGCGGGGTATCAGCTTTTTTCCCCCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGc < 1:345784/90‑1 (MQ=255) ttGTGTGCCGGTTATCAGCGTTATTCCCCCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGc < 1:49369/90‑1 (MQ=255) ttGTGTGCCGGGTATCAGCTTTTTTCCCCCCATCTCCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGc < 2:23916/90‑1 (MQ=255) ttGTGGGCGGGGTTTCAGCTTTTTTCCCCCCATCTCCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGc < 2:206248/90‑1 (MQ=255) tgtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGAc > 1:294335/1‑90 (MQ=255) tgtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGAc > 1:480715/1‑90 (MQ=255) tgtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGAc > 1:411809/1‑90 (MQ=255) tCAGCGTTTTTCCCCCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTatgcatgc < 1:610983/90‑1 (MQ=255) tATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTCGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTg > 1:223477/1‑90 (MQ=255) | TGGGGAGGCTGCCCGAAACACCGCTTCATGCTCGATGCCAGCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTG > NZ_CP009273/3977362‑3977535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |