| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 228,191 | 0 | A | G | 53.3% | 6.6 / 15.8 | 16 | S195G (AGC→GGC) | yafD | endonuclease/exonuclease/phosphatase family protein |
| Reads supporting (aligned to +/- strand): ref base A (1/6); new base G (8/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAG‑AAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGA > NZ_CP009273/228104‑228261 | gTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGc < 2:528991/90‑1 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAATTCCTGGGGCCCGAG‑aaag > 1:627596/1‑88 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAATGCCTGGGGGCGGAG‑AAgg > 1:10619/1‑90 (MQ=255) aGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTTAATTCCCTGGGCCGGag‑gaggg > 2:205028/1‑89 (MQ=255) gTTACTTCCTATTGGCGATCAGATAGCTCCCCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAG‑AAGGATGaa < 1:493922/90‑1 (MQ=255) gTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTTATGTCCTGTGGCGTAGAAAGGATGa > 1:339772/1‑90 (MQ=255) ttCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAG‑AAGGATGAACGCGt < 1:281744/90‑1 (MQ=255) tAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTTATGCCTGGGGCCGTAG‑AAGGATGAAAGCGGTATATTGCTTTTCGCggg > 1:213103/1‑90 (MQ=255) tCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTTTATGCCTGGGGCCGTAG‑AAGGGTGAAAGCGGTTTATTGCTTTGCGCGGGgaaa > 1:64926/1‑89 (MQ=255) ccacAGCGGCCCGGTCATTATGGCGGGGGGTTTTAAAGCCTGGGGCCGGAG‑GAGGGGGAAAGCGGTATATTGCTTTTTGCGGGGAAAGtg > 2:217131/1‑89 (MQ=255) gCGGCCCGGTCATTATGGCGGGGGATTTTAAAGCCTGGGGCCGTAG‑AAGGAAGAACGCGGTTTATTTCTTTTCGCGGGGAAAGGCGCTgc > 1:307645/1‑90 (MQ=255) ccGGTCATTATGGCGGGGGATTTTAACGCCTGGGGCCGTAG‑AAGGAAGAAAGCGGTTTATTGCTTTTCGCGGGGAATGTCGCTGCGcccg > 1:567965/1‑88 (MQ=255) cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAG‑AAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAgg < 1:171949/90‑1 (MQ=255) ggTCATTATGGCGGGGGGTTTCAATGCCTGGAGCCGTAG‑AAGGGGGAACGCGTTATATCGCTTTGCGCGGGGAATGTCGCTGCGCCAggg > 2:578820/1‑89 (MQ=255) gggAGATTTCAATGCCTGGAGCCGTAG‑AAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCga < 1:545509/90‑1 (MQ=255) gggAGATTTCAACGCCTGGAGCCGTAG‑AAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCga < 2:567965/90‑1 (MQ=255) | GTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAG‑AAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGA > NZ_CP009273/228104‑228261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |