Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 49,905 T→G L28R (CTC→CGC)  folA → type 3 dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927349,9050TG91.9% 109.8 / 1.4 37L28R (CTC→CGC) folAtype 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (13/21);  total (14/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49820‑49991
                                                                                      |                                                                                      
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    <  1:17787/90‑1 (MQ=255)
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    <  1:499379/90‑1 (MQ=255)
  aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGACCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTg                                                                                  <  2:360714/90‑1 (MQ=255)
       tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTa                                                                             <  1:153387/90‑1 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaa                                                                            >  1:149097/1‑90 (MQ=255)
          gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAAc                                                                          <  2:275826/90‑1 (MQ=255)
             tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGGTTAAACGCa                                                                       >  2:310805/1‑90 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCTTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:497276/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:319612/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:148703/90‑1 (MQ=255)
               aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac                                                                     <  2:382613/90‑1 (MQ=255)
               aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac                                                                     <  2:528066/90‑1 (MQ=255)
               aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac                                                                     <  1:219700/90‑1 (MQ=255)
               aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac                                                                     <  2:295681/90‑1 (MQ=255)
               aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac                                                                     <  2:212435/90‑1 (MQ=255)
                   cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAACCGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCt                                                                 <  2:481065/90‑1 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  2:6543/1‑90 (MQ=255)
                        ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaaat                                                            >  2:16157/1‑90 (MQ=255)
                               tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                                     <  1:394764/90‑1 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTTGAACCTGCCTGCCGATCGCGCCTGGTTTAAACCCAACACCTTAAATAAACCCGTGATTa                                               >  2:579851/1‑90 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                               <  2:119/90‑1 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaat                                                            >  1:151659/1‑74 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaat                                                            <  2:151659/74‑1 (MQ=255)
                                         tacgggCATGAAAAACGCCATGCCGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                           <  2:288713/86‑1 (MQ=255)
                                               gcaggAAAACCCCCATGCCGTGAAACCTCCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcc                                      <  2:613032/87‑1 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      >  2:231013/1‑56 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:231013/56‑1 (MQ=255)
                                                        cGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                            >  2:1373/1‑90 (MQ=255)
                                                             aTGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                        >  2:181136/1‑90 (MQ=255)
                                                             aTGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                        >  2:160554/1‑90 (MQ=255)
                                                              tGCCGTGGAACCTGCCTGCCGATCGCGCCTTGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                           <  1:301707/70‑1 (MQ=255)
                                                              tGCCGTGGAACCTGCCTGCCGATCGCGCCTTGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                           >  2:301707/1‑70 (MQ=255)
                                                              tGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                       >  1:396092/1‑90 (MQ=255)
                                                                  gTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg                   >  2:444424/1‑90 (MQ=255)
                                                                       aCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc              <  2:332190/90‑1 (MQ=255)
                                                                          tgcctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt           >  2:587399/1‑90 (MQ=255)
                                                                                   aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  2:396092/90‑1 (MQ=255)
                                                                                      |                                                                                      
TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49820‑49991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: