Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	579,943	(T)8→7	intergenic (+57/+193)	appY → / ← ompT	DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	579,936	0	T	.	100.0%	28.3 / NA	8	intergenic (+50/+200)	appY/ompT	DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/4);  total (4/4)

GATGGAATAAACAAAACAATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACT  >  NZ_CP009273/579860‑580005                                                                             |                                                                      gATGGAATAAACAAAACAATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGa                                                         <  2:359521/90‑1 (MQ=255)               aacaaTTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCAt                                           >  1:125447/1‑90 (MQ=255)                   aTTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAAt                                       >  2:489414/1‑90 (MQ=255)                                         aaGTTGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGcc                 <  2:125447/90‑1 (MQ=255)                                         aaGTTGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGcc                 <  2:140217/90‑1 (MQ=255)                                                     tGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTatacat     <  1:490528/90‑1 (MQ=255)                                                     tGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTatacat     >  2:490528/1‑90 (MQ=255)                                                        tAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACt  >  2:200073/1‑90 (MQ=255)                                                                             |                                                                      GATGGAATAAACAAAACAATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACT  >  NZ_CP009273/579860‑580005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑