Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,417,788	(C)6→7	coding (69/120 nt)	BW25113_RS26130 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,417,782	1	.	C	92.3%	30.1 / ‑2.0	13	coding (75/120 nt)	BW25113_RS26130	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (2/10);  total (2/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACT  >  NZ_CP009273/1417716‑1417858                                                                    |                                                                             cacaTCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGc                                                        <  1:549540/90‑1 (MQ=255)       aaaGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACg                                                  <  1:46779/90‑1 (MQ=255)           cAGTCTGTCAGTCAGTGCGTGAAGCCCCCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGat                                              <  2:335618/90‑1 (MQ=11)                   cagtcagtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCAc                                      <  2:175512/90‑1 (MQ=255)                             gTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGaa                            <  2:543307/90‑1 (MQ=255)                                  gCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACt                       <  1:505045/90‑1 (MQ=255)                                       caccGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTaa                  <  1:294304/90‑1 (MQ=255)                                           gCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAatgat              >  1:9445/1‑90 (MQ=255)                                                  gCGTGGATAATGCCGCCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc      <  1:298401/90‑1 (MQ=255)                                                   cGTGGATAATGCAGCCTCCCCCCCGGCTGGCAGACCCCGCTGACCGGGATTATTTCACCCTCCGGGAACGCCTGGCACTGATGCAGGccc      <  1:536052/90‑1 (MQ=17)                                                   cGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc      <  1:481476/90‑1 (MQ=255)                                                   cGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc      <  2:557521/90‑1 (MQ=255)                                                       gATAATGCAGCCTCCCCCCCGACTGGCAGACGCCGCTGAACGGAATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACt  >  2:203318/1‑90 (MQ=21)                                                                    |                                                                             CACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACT  >  NZ_CP009273/1417716‑1417858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑