| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 40,515 | 0 | G | T | 62.5% | 6.1 / 18.0 | 16 | Q473K (CAA→AAA) | caiT | L‑carnitine/gamma‑butyrobetaine antiporter |
| Reads supporting (aligned to +/- strand): ref base G (0/6); new base T (10/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCAC > NZ_CP009273/40431‑40590 | tCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGGTTTTATCg > 1:463611/1‑90 (MQ=255) gcgTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAgg > 2:8597/1‑90 (MQ=255) tttAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTTGggcgcc > 2:478584/1‑90 (MQ=255) gagaGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCcagc < 2:15897/90‑1 (MQ=255) gagCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATTGGGTTTAGGCCGCCCGGCGCccgcag > 2:406525/1‑90 (MQ=255) gagCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAGGCCGCCGGGCGCccgcag > 2:8243/1‑90 (MQ=255) gCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAAGGCGCCGGGGGCccgccaaa > 2:216879/1‑86 (MQ=255) cGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAAc < 1:478584/90‑1 (MQ=255) cGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAAc < 2:323403/90‑1 (MQ=255) aCCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAGGCCGCCCGGGGCCCGCAGAaaaaaa > 2:497197/1‑88 (MQ=255) aTGTTGACGGAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAGGCCGCCGAGCGCCAGCAGAACAAAAACGATa > 2:426592/1‑90 (MQ=255) gACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGTTTTAGGCCGCCGGGCGCCCGCAGAACAATACCGATAATGcc > 2:213558/1‑90 (MQ=255) gACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAGGCCGCCGAGCGCCCGCAGAACAAAAACGATAATGcc > 2:40326/1‑90 (MQ=255) gggCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGAc < 2:171225/90‑1 (MQ=255) gCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCa < 2:475759/90‑1 (MQ=255) gATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCAc < 2:463611/90‑1 (MQ=255) | TCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCAC > NZ_CP009273/40431‑40590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |