| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 40,525 | 0 | C | T | 60.0% | ‑2.3 / 11.5 | 15 | L469L (CTG→CTA) | caiT | L‑carnitine/gamma‑butyrobetaine antiporter |
| Reads supporting (aligned to +/- strand): ref base C (0/6); new base T (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCAC > NZ_CP009273/40439‑40590 | gcgTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAgg > 2:8597/1‑90 (MQ=255) tttAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTTGggcgcc > 2:478584/1‑90 (MQ=255) gagaGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCcagc < 2:15897/90‑1 (MQ=255) gagCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATTGGGTTTAGGCCGCCCGGCGCccgcag > 2:406525/1‑90 (MQ=255) gagCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAGGCCGCCGGGCGCccgcag > 2:8243/1‑90 (MQ=255) gCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAAGGCGCCGGGGGCccgccaaa > 2:216879/1‑86 (MQ=255) cGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAAc < 1:478584/90‑1 (MQ=255) cGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAAc < 2:323403/90‑1 (MQ=255) aCCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAGGCCGCCCGGGGCCCGCAGAaaaaaa > 2:497197/1‑88 (MQ=255) aTGTTGACGGAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAGGCCGCCGAGCGCCAGCAGAACAAAAACGATa > 2:426592/1‑90 (MQ=255) gACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGTTTTAGGCCGCCGGGCGCCCGCAGAACAATACCGATAATGcc > 2:213558/1‑90 (MQ=255) gACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAGGCCGCCGAGCGCCCGCAGAACAAAAACGATAATGcc > 2:40326/1‑90 (MQ=255) gggCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGAc < 2:171225/90‑1 (MQ=255) gCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCa < 2:475759/90‑1 (MQ=255) gATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCAc < 2:463611/90‑1 (MQ=255) | GCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCAC > NZ_CP009273/40439‑40590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |