| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 595,037 | 0 | A | G | 60.0% | 6.2 / 12.4 | 15 | I290V (ATC→GTC) | cusA | Cu(+)/Ag(+) efflux RND transporter permease subunit CusA |
| Reads supporting (aligned to +/- strand): ref base A (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGAT‑CCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCT > NZ_CP009273/594961‑595113 | ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGT‑CCTGCGGTcccg > 2:344544/1‑88 (MQ=255)ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGT‑CCTGCGGTCCgg > 2:135056/1‑90 (MQ=255)ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGAT‑CCTTCGATCCgg > 2:190505/1‑90 (MQ=255) tCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGT‑CCCGCGGTCCCGCa > 2:571940/1‑90 (MQ=255) aTTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGAT‑CCTGCGATCCGGCAAAAAc < 1:110747/90‑1 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTGCCGGCG‑GCCGGCCAAAACGcc > 1:40774/1‑90 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGT‑CCCCCGCCCCGGCGAAAAAGcc > 1:17795/1‑90 (MQ=255) ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGAT‑CCTGCGATCCGGCAAAAACGCCCgaga < 2:189651/90‑1 (MQ=255) gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGT‑CCTGCGATCCGGCAAAAAAGCCCCAGAAGGGAt > 2:583414/1‑90 (MQ=255) aCTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGAT‑CCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaa < 2:6907/90‑1 (MQ=255) aaaCGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGT‑CCTTCGAACCGGCAAAAAACCCCGGGAAGGGATGGCCGCCGTGGAGGAaaaaac > 1:291266/1‑88 (MQ=255) aaaCGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGT‑CCTGCGATCCGGCAAAAAAGCCCGAGAAGGGAGGGCCGCCGGGGAGGAGAAACt > 2:491100/1‑90 (MQ=255) gcgaagTGGCGGGCGGGGTGGTGAT‑CCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGa < 2:249387/90‑1 (MQ=255) gcggggggGGGGGGGGT‑GCCGCGATCCGGCAAAAAAAACCGAGAAGGGGTTGCCGCCGGGGAGGGCAAAATGGAAAAGATGAAAaatagc > 1:551582/1‑89 (MQ=255) gggcgggGTGGTGAT‑CCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCt < 2:291266/90‑1 (MQ=255) | GGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGAT‑CCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCT > NZ_CP009273/594961‑595113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |