| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,500,285 | 0 | A | C | 61.5% | 5.1 / 11.0 | 13 | V18G (GTA→GGA) | ydcO | BenE family transporter YdcO |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/8); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGC > NZ_CP009273/1500205‑1500366 | cagAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGaa > 1:81932/1‑90 (MQ=255) gagaTTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAAc > 1:187210/1‑90 (MQ=255) agttttGTGCGGGGGTGGCTCCGGCGAAAATCGCTCCTTGCCAGATTATTGCCGCTGAACGGGCGTAGCCAATTAACCCCGCCA‑AAAccc < 1:176186/87‑1 (MQ=255) ttGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGc > 2:238352/1‑90 (MQ=255) tgtgCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGcc > 1:38183/1‑90 (MQ=255) ggCGACAACCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCAAATTAATCCCGCCAGAACCCCCGCCAGTAGCGTGGGTggagg < 2:1333/90‑1 (MQ=255) ggCAACAACCGCTCCTTCCAAATTTTTTCCCGCTGAACGGGGGAACCAAATAAACCCCCCCAAAACCCCCGCCAGTAGCGTGGGTggagg < 1:71409/90‑1 (MQ=255) gctgttTGCAAGTTTTTTGCCGCTGAACGGGCGTAGCAAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAAc < 2:541125/90‑1 (MQ=255) cctgctgGCCAGTTTATGGCCGGTGAAGGGGGGAAGCAAATAAACCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAAc < 1:569073/89‑1 (MQ=255) cgggCGTAGCAAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtata < 2:253623/88‑1 (MQ=255) ggCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGc > 2:568684/1‑90 (MQ=255) tgCCATTTAACCCCGCGGGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACa < 2:369177/89‑1 (MQ=255) aaaTAAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGc < 2:444790/89‑1 (MQ=255) | CAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGC > NZ_CP009273/1500205‑1500366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |