| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,630,518 | 0 | A | C | 60.0% | 11.5 / 15.8 | 15 | G66G (GGT→GGG) | der | ribosome biogenesis GTPase Der |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/9); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTC > NZ_CP009273/2630429‑2630601 | aTCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTaca < 1:83823/90‑1 (MQ=255) aaCAGTCCGCCTCCCGCTCCTCCAACCCCCACCAGCGCCTGTCCCCCCAGGGGGTTTTCTACCCCGTCTTCTGTGCCATCAATCccgccg < 1:78817/90‑1 (MQ=255) aaCAGTCCGCCGCCCTTTTCTCCAACCCCCAGCGCCGACTGTCCCGCCATGGGGGTTTCTACCCCGTTTTCTGTGCCATCAACCccgccg < 1:196990/90‑1 (MQ=255) aaCAGTACGCCGCCCCTTTCTTCAACCCCCAGCAGCGACTGTCCCCCCATGGGGTTTTCTACCCCGTCTTCTGTGCCATCAACCccgccg < 1:163319/90‑1 (MQ=255) gTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTAt > 1:339403/1‑90 (MQ=255) gTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTAt > 1:536698/1‑90 (MQ=255) tACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATc > 2:78817/1‑90 (MQ=255) gacgCCCGTTTCTCAAACCCCCAGCAGCGCCTTTCCCCCCAGGGGGGTTTCTACCCCGTCTTCTGTGCCATAAACCCCGCCGGTATCAAt < 1:64753/90‑1 (MQ=255) acgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATa > 2:52140/1‑90 (MQ=255) cgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACCCCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATAc < 2:2761/90‑1 (MQ=255) cGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGAt > 1:411134/1‑90 (MQ=255) cttcttCAATCCCCACCACCGCCTTTCCCCCCATGGGGTTTTCCACCCCTTCTCCTGCGCCATCAATCCCGCCGGTATCAATACAGATaa < 1:332601/90‑1 (MQ=255) cccccAGCCGCGACTTTCCCGCCATGCGGTTTTCTACCCCTTCTTCTGTCCCACCAACCCCGCCGGTATCAATACAGATAAACTCACGGc < 1:547696/87‑1 (MQ=255) agcGATTTTCCCGCCATGCGGGTTTCTCCCCCTTCTTCTGTGCCATCAACCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAAtt < 2:519828/90‑1 (MQ=255) ggttttCTCCCCCGTCTTCTGTGCCATCAACCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTAc < 1:115677/87‑1 (MQ=255) ttCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTc > 2:436370/1‑90 (MQ=255) | ATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTC > NZ_CP009273/2630429‑2630601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |