| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,955,854 | 0 | T | G | 57.1% | 14.5 / 20.2 | 21 | S90S (TCA→TCC) | ppdC | prepilin‑type N‑terminal cleavage/methylation domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base G (12/0); total (15/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGC > NZ_CP009273/2955772‑2955942 | ggTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTTCCCCCGGGGGAAACtg > 2:294336/1‑89 (MQ=255)ggTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTa > 2:536713/1‑90 (MQ=255)ggTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTa > 1:249348/1‑90 (MQ=255) aCCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTa < 1:132876/90‑1 (MQ=255) ccTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTaaa > 2:253301/1‑89 (MQ=255) tAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCt > 2:351971/1‑90 (MQ=255) tAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCGCCCTTTCTTCCCCCCGGGGGAAATAACGGAAAGCt > 1:429566/1‑90 (MQ=255) tAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGCCATCTCTCCCTCTCTGCCCCCGGGGGAAACTAAGGTaaaccc > 1:86892/1‑86 (MQ=255) aGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCCCCTTCTCTGCCCCCGGGGGAAACTAGCCTAACGCTGATGCt > 2:420053/1‑90 (MQ=255) aGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCGCCCTCTCTGCCCCCCGGGGGAAATAGCGTAACGCTGATGCt > 1:358514/1‑90 (MQ=255) aGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCCCCTTCTCTGCCCCCCGGGGAAACTAGCGTAACGCCGGTGCt > 1:278083/1‑90 (MQ=255) gACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGGAACTACCGGAAAGCTGGTGCTGACACATCCCGCCTGCg > 1:198215/1‑90 (MQ=255) gACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCCCCTGCg > 1:213205/1‑90 (MQ=255) aCGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGa < 2:249348/90‑1 (MQ=255) ttCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTc < 2:429522/90‑1 (MQ=255) ttCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTc < 2:429566/90‑1 (MQ=255) aaTGCAGGCGGGTCATCTCCCCTTCTCTGCCCCCGGGTGAAACTAGCGGAACGCCGATGCTTACACATCCCCCCTTCGATGTCTGCATTc > 2:327380/1‑90 (MQ=255) ggCGGGGCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGa > 1:314866/1‑90 (MQ=255) tctgTGCCCCCGGGTGAAACTAGCGTACCGTTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGt < 1:294336/90‑1 (MQ=255) gggTGGAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATcgc > 1:442997/1‑90 (MQ=255) gTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATcgcgc < 1:137927/90‑1 (MQ=255) | GGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGC > NZ_CP009273/2955772‑2955942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |