| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,502,021 | 0 | T | A | 57.1% | ‑2.2 / 16.1 | 14 | E384D (GAA→GAT) | yhfW | phosphopentomutase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base A (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGAT > NZ_CP009273/3501933‑3502094 | gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAtt < 1:341239/90‑1 (MQ=255) cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACCGTAGcc > 2:355623/1‑90 (MQ=255) cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGGAAAAATTACACACGGGAGcc > 2:554248/1‑90 (MQ=255) gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAAATTACACACGGGAGCCCCCCCATc > 2:346945/1‑90 (MQ=255) gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAAATCACACACCGCAGGCCCCCCATc > 1:86392/1‑90 (MQ=255) gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACACACGGGAGCCCCCCCATc > 2:37041/1‑90 (MQ=255) aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCacac < 1:518150/90‑1 (MQ=255) agaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc < 1:415440/90‑1 (MQ=255) agaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc < 2:445680/90‑1 (MQ=255) agaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc < 2:513964/90‑1 (MQ=255) agCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACCGGAGCCCCCCCATCAGAAAACGGGGGGGGCCCACCCAGCTGCGGAg > 2:331838/1‑90 (MQ=255) agCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg < 1:219706/90‑1 (MQ=255) cGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACAAACGGGAGCCCCCCCATCAAAAAACGGGGGGGGCACACCCAGCTGCGTTGCg > 2:120545/1‑90 (MQ=255) aCCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACCGGAGCCCCCCCCTCAGAAAACGGGGGGGGGCCACCCCGCCGCGTAGCGat > 1:208340/1‑90 (MQ=255) gtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGAt < 2:338604/90‑1 (MQ=255) | GTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGAT > NZ_CP009273/3501933‑3502094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |