| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 61.9% | ‑6.5 / 15.8 | 21 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (8/0); new base A (0/13); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAG > NZ_CP009273/4058879‑4059037 | ttAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATc > 1:534544/1‑90 (MQ=255) ttttcCCGGGAGTCGAGAAAAAACCCGGTGAGCATATCGGTAACCGCGGAAAAAAACTTCCCCACCAAAAAGATAATCCCGCCATAGTaa < 2:62663/90‑1 (MQ=255) ttttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAg > 2:366432/1‑90 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa > 1:528058/1‑90 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa > 1:553013/1‑90 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa > 2:132483/1‑90 (MQ=255) agaaaaaaCCCGGTGAGCATATCGGAAAACGCGGAAAAAAACTCCCCCACCAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAAt < 2:5277/87‑1 (MQ=255) aaaCCCGGGGAGCATATCGGAAACCGCGAAAAAAACCTCCGCCACAAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:553505/90‑1 (MQ=255) ggTGAGAATATGGGTAAACGCGAAAAAAAACTTCCCCACAAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 2:528058/90‑1 (MQ=255) gCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAtt > 2:551284/1‑90 (MQ=255) tatCGAAAACCGCGAAAAAAACCTCCCCCACCAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga < 1:31508/90‑1 (MQ=255) gaaaaCGCGGAAAAAAACTCCCCCACAAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga < 2:89233/88‑1 (MQ=255) gTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa > 1:85115/1‑58 (MQ=255) gTAAACGCGGTAAAAAACTTCGCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCa < 2:85115/58‑1 (MQ=255) tAAACGCGAAAAAAATCTCCCCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGAt < 1:289263/90‑1 (MQ=255) aaCGCGAAAAAAACCTCCGCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATAc < 1:574466/90‑1 (MQ=255) ggTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGt > 2:273978/1‑90 (MQ=255) aaaaaaaCTCCCCCAAAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAc < 1:405757/89‑1 (MQ=255) aaaaacCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAcc < 1:519211/90‑1 (MQ=255) tcccccacaaAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 2:258161/86‑1 (MQ=255) ccccccacaaAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 1:457918/86‑1 (MQ=255) | TTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAG > NZ_CP009273/4058879‑4059037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |