| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,142,237 | 0 | T | G | 57.1% | 5.4 / 10.5 | 14 | T264P (ACC→CCC) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (8/0); total (11/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGCAGTTTGT > NZ_CP009273/4142159‑4142326 | gTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAg > 1:200346/1‑90 (MQ=255)gTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAg > 2:156256/1‑90 (MQ=255) cAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGGTATCGGCAGTGACGTTCgg > 2:294903/1‑90 (MQ=255) gCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGTAGGACGTGGCGGGGGATATCGGCAGGGACGTTCgggg > 2:160811/1‑89 (MQ=255) gCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGACGTTCgggg > 2:8784/1‑89 (MQ=255) tctCTTTCAGCAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGCCGTGGGGGATGATATCGGAAGTGACGATCGGGTTGCCGTcgc < 1:336315/88‑1 (MQ=255) tttCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGACGTTCGGGGTGCCGTCGCgggc > 2:410009/1‑88 (MQ=255) ggAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACGTTCGGGTTGCCGTCGCGGTcgccgc < 1:535224/90‑1 (MQ=255) ggAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACGTTCGGGTTGCCGTCGCGGTcgccgc < 2:200346/90‑1 (MQ=255) gTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGGTATCGGCAGGGGCGTTCGGGGTGCCGGCGCGGGCGCCCCCCCTCCCCGaa > 1:365476/1‑90 (MQ=255) gTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTGACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGaa > 2:149971/1‑90 (MQ=255) gCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGGCGTTTGGGGTGCCGTCGCGGGCGCCGCCCATCCCCGAAgga > 1:244164/1‑88 (MQ=255) ggCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACGTTCGGGTTGCCGTCGCGGTCGCCCCCCCTCCACGAAGTAAAACGGACCg > 2:34996/1‑90 (MQ=255) gCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGACGTTCGGGGTGGCGTCGCGGGCGCCGCCCCTCCACGAAGTAAAAAGGACCgg > 1:1827/1‑90 (MQ=255) tGATATCGGCAGTGACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGCAGTTTGt < 2:126197/90‑1 (MQ=255) | GTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGCAGTTTGT > NZ_CP009273/4142159‑4142326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |