Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,122,267	A→C	S35A (TCG→GCG)	wcaF ←	colanic acid biosynthesis acetyltransferase WcaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,122,267	0	A	C	100.0%	31.5 / NA	11	S35A (TCG→GCG)	wcaF	colanic acid biosynthesis acetyltransferase WcaF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (2/9);  total (2/9)

CTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAG  >  NZ_CP009273/2122180‑2122349                                                                                        |                                                                                   ccgacggacgAATAACTGCGTTTTTTCCTATTCTTCCTCCGAAAAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGccc                                                                                  <  1:465682/88‑1 (MQ=255)      ggacgAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCa                                                                             <  2:386002/90‑1 (MQ=255)      ggacgAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCa                                                                             <  2:54383/90‑1 (MQ=255)             tAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                                                                      <  1:573939/90‑1 (MQ=255)                  aCGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGcc                                                                 <  1:461791/90‑1 (MQ=255)                  aCGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGcc                                                                 <  1:79740/90‑1 (MQ=255)                  aCGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGcc                                                                 <  2:512103/90‑1 (MQ=255)                  aCGTTTTTTCCTATTTTTGCTCCGAATAAACGTAAAAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGcc                                                                 <  1:311453/90‑1 (MQ=255)                       ttttCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTAc                                                            <  2:387715/90‑1 (MQ=255)                                        cGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCa                                           >  1:299719/1‑90 (MQ=255)                                                                 ccAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGaa                  <  1:340304/90‑1 (MQ=255)                                                                                 tgtgGCGCCCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCgag  >  2:149164/1‑90 (MQ=255)                                                                                        |                                                                                   CTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAG  >  NZ_CP009273/2122180‑2122349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑