Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,131,004	A→G	intergenic (‑280/‑379)	wza ← / → yegH	polysaccharide export protein Wza/TerC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,131,004	0	A	G	100.0%	51.5 / NA	17	intergenic (‑280/‑379)	wza/yegH	polysaccharide export protein Wza/TerC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/7);  total (10/7)

TTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGG  >  NZ_CP009273/2130923‑2131088                                                                                  |                                                                                     tttAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACt                                                                              <  2:316488/90‑1 (MQ=255)                  tGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGAc                                                             >  1:132371/1‑90 (MQ=255)                   gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                            >  1:315410/1‑90 (MQ=255)                   gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                            >  2:403296/1‑90 (MQ=255)                             aaTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAAt                                                  >  1:520260/1‑90 (MQ=255)                                      cacCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTg                                         <  1:233763/90‑1 (MQ=255)                                           tctcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCttt                                    <  2:580063/90‑1 (MQ=255)                                                  aTGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaataa                             >  1:475755/1‑90 (MQ=255)                                                  aTGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaataa                             >  1:372892/1‑90 (MQ=255)                                                  aTGCAGTCGTTGATGAGAAAGGGTTATTAAGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaataa                             >  1:425951/1‑90 (MQ=255)                                                     cAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGc                          <  2:3912/90‑1 (MQ=255)                                                            tgatgaGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAggagga                   >  1:475228/1‑90 (MQ=255)                                                              atgaGAAAGGGTTATTACGGGAATTACCTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAg                 <  2:286662/90‑1 (MQ=255)                                                                    aaGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTAc           >  1:121731/1‑90 (MQ=255)                                                                         ttattaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTa      >  1:373739/1‑90 (MQ=255)                                                                           attaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAAc    <  2:373739/90‑1 (MQ=255)                                                                             taCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACgg  <  2:315410/90‑1 (MQ=255)                                                                                  |                                                                                     TTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGG  >  NZ_CP009273/2130923‑2131088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑