Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,751,237 A→G V241V (GTT→GTC)  BW25113_RS13685 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,751,2370AG90.9% 23.7 / ‑3.0 11V241V (GTT→GTC) BW25113_RS13685hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (5/5);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

CTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTC  >  NZ_CP009273/2751158‑2751312
                                                                               |                                                                           
ctGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCat                                                                   >  2:114744/1‑90 (MQ=255)
                          ggTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGa                                         >  1:521513/1‑90 (MQ=255)
                           gTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGaa                                        >  2:401702/1‑90 (MQ=255)
                            ttCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAAt                                       <  1:186158/90‑1 (MQ=255)
                            ttCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAAt                                       <  1:222338/90‑1 (MQ=255)
                            ttCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAAt                                       <  2:498218/90‑1 (MQ=255)
                                 tAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCGTTAATTAGCTCAACCAACTTCAGTTGAATGatat                                  <  1:402845/90‑1 (MQ=255)
                                    gTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGt                               >  2:131565/1‑90 (MQ=255)
                                              tGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgc                     >  1:305506/1‑90 (MQ=255)
                                                            ttttCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCAc       <  2:264306/90‑1 (MQ=255)
                                                                 cACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCcttc  <  2:521513/90‑1 (MQ=255)
                                                                               |                                                                           
CTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTC  >  NZ_CP009273/2751158‑2751312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑