Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,753,169	T→C	S276P (TCA→CCA)	yfjI →	YfjI family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,753,169	0	T	C	100.0%	55.4 / NA	18	S276P (TCA→CCA)	yfjI	YfjI family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/10);  total (8/10)

AAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTTCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATT  >  NZ_CP009273/2753084‑2753237                                                                                      |                                                                     aaaGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCaaa                                                                  <  2:182192/90‑1 (MQ=255)       gattggattTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACa                                                            >  2:520070/1‑90 (MQ=255)       gattggattTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACa                                                            >  1:361903/1‑90 (MQ=255)        attggattTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACAt                                                           >  2:281769/1‑90 (MQ=255)             attTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGcc                                                      <  2:441428/90‑1 (MQ=255)              ttttttGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCg                                                     <  2:406042/90‑1 (MQ=255)                          tGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                         <  2:93456/90‑1 (MQ=255)                          tGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                         <  2:135375/90‑1 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGt                                                   <  2:308318/71‑1 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGt                                                   >  1:308318/1‑71 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                >  2:245517/1‑90 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                >  1:23703/1‑90 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                >  1:135702/1‑90 (MQ=255)                                        gCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAAt                           <  2:135702/90‑1 (MQ=255)                                        gCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAAt                           <  1:213856/90‑1 (MQ=255)                                           tGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTCCCGGTATTTCACCAACGTCTTATGGAAATTGt                        <  1:513557/90‑1 (MQ=255)                                                     caAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGc              <  2:252044/90‑1 (MQ=255)                                                                 aaaaTTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAAtt  >  1:440890/1‑90 (MQ=255)                                                                                      |                                                                     AAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTTCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATT  >  NZ_CP009273/2753084‑2753237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑