Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,887,405	C→A	P43Q (CCG→CAG)	ygcO →	ferredoxin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,887,405	0	C	A	100.0%	72.3 / NA	20	P43Q (CCG→CAG)	ygcO	ferredoxin family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/14);  total (6/14)

ACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCCGTTTGCTGTGCGACGAATCAAC  >  NZ_CP009273/2887321‑2887480                                                                                     |                                                                            acaTTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAg                                                                        <  1:49896/90‑1 (MQ=255)         ccGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTa                                                                <  1:363897/90‑1 (MQ=255)              tGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTAt                                                           <  1:542706/90‑1 (MQ=255)                aCTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGCATTg                                                         >  2:399430/1‑90 (MQ=255)                          gcgcCAGACGGCAGAGCGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAg                                               <  1:285939/90‑1 (MQ=255)                          gcgcCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAg                                               <  1:548877/90‑1 (MQ=255)                          gcgcCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAg                                               <  1:542494/90‑1 (MQ=255)                                  cGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTgg                                       <  1:193031/90‑1 (MQ=255)                                  cGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTgg                                       <  1:330760/90‑1 (MQ=255)                                  cGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGAATTGACTATCGCAGTTGCCTgg                                       <  1:399430/90‑1 (MQ=255)                                       gAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAgtgt                                  >  2:74778/1‑90 (MQ=255)                                       gAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAgtgt                                  >  2:39861/1‑90 (MQ=255)                                             ttGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCAcc                            <  1:490577/90‑1 (MQ=255)                                                      gCCTGTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCCGTTTg                   >  1:218602/1‑90 (MQ=255)                                                          gTCCGGCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCCGTTTGCtgt               >  1:462035/1‑90 (MQ=255)                                                               gCAGGTCTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCCGTTTGCTGTGcgac          >  1:297061/1‑90 (MQ=255)                                                                     cTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCCGTTTGCTGTGCGACGAATCa    <  2:29315/90‑1 (MQ=255)                                                                     cTTTTTTCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCCGTTTGCTGTGCGACGAATCa    <  2:297061/90‑1 (MQ=255)                                                                     cTTTTTTCACTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCCGTTTGCTGTGCGACGAATCa    <  1:549980/90‑1 (MQ=255)                                                                       tttttCGCTCACACAGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCCGTTTGCTGTGCGACGAATCAac  <  2:313094/90‑1 (MQ=255)                                                                                     |                                                                            ACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCCGTTTGCTGTGCGACGAATCAAC  >  NZ_CP009273/2887321‑2887480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑