Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,002,903	T→C	S261P (TCA→CCA)	ygeY →	YgeY family selenium metabolism‑linked hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,002,903	0	T	C	100.0%	82.4 / NA	26	S261P (TCA→CCA)	ygeY	YgeY family selenium metabolism‑linked hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/11);  total (15/11)

TCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGA  >  NZ_CP009273/3002823‑3002981                                                                                 |                                                                               tCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGAcc                                                                       >  1:178113/1‑90 (MQ=255)  cGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACcg                                                                      >  1:530775/1‑90 (MQ=255)       aaGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCgtcgtc                                                                 >  1:366795/1‑90 (MQ=255)             cccTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCt                                                           >  1:125491/1‑90 (MQ=255)                   ccGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCg                                                     >  2:574557/1‑90 (MQ=255)                    cGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGa                                                    <  1:128509/90‑1 (MQ=255)                       ttCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACcg                                                                      <  2:92658/69‑1 (MQ=255)                       ttCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACcg                                                                      >  1:92658/1‑69 (MQ=255)                                 ttcttcACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGgc                                       <  1:50305/90‑1 (MQ=255)                                          tCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGAc                              >  1:156100/1‑90 (MQ=255)                                                    gTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCcgcg                    >  2:12630/1‑90 (MQ=255)                                                     ttGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCgcgc                   >  1:542889/1‑90 (MQ=255)                                                       gcgcTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGccc                 <  1:410101/90‑1 (MQ=255)                                                       gcgcTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGccc                 <  2:275136/90‑1 (MQ=255)                                                         gcTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctg               <  2:328501/90‑1 (MQ=255)                                                         gcTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctg               <  1:68807/90‑1 (MQ=255)                                                               gCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCa         <  1:143041/90‑1 (MQ=255)                                                                cAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAg        <  1:393707/90‑1 (MQ=255)                                                                 aGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGt       >  1:361499/1‑90 (MQ=255)                                                                    cAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACa    >  1:199603/1‑90 (MQ=255)                                                                    cAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACa    >  2:305234/1‑90 (MQ=255)                                                                     aGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAg   <  1:51247/90‑1 (MQ=255)                                                                      gCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGGAACCTGGGAAGGCGCGCTGGACGa                            >  2:214633/1‑64 (MQ=255)                                                                      gCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGa                            <  1:214633/64‑1 (MQ=255)                                                                      gCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGa  >  2:188599/1‑90 (MQ=255)                                                                      gCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGa  >  1:377103/1‑90 (MQ=255)                                                                                 |                                                                               TCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGA  >  NZ_CP009273/3002823‑3002981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑