Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,786,467	T→C	I193V (ATT→GTT)	yibB ←	protein YibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,786,467	0	T	C	100.0%	129.9 / NA	39	I193V (ATT→GTT)	yibB	protein YibB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/13);  total (26/13)

AATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGG  >  NZ_CP009273/3786394‑3786552                                                                          |                                                                                      aaTTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAAcc                                                                       >  2:360401/1‑90 (MQ=255)    ttATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCtgt                                                                    <  2:491010/90‑1 (MQ=255)        aGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTgttgtt                                                                <  2:339267/90‑1 (MQ=255)                ttCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGAtgtg                                                        >  1:361621/1‑90 (MQ=255)                     tttATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGt                                                   >  2:339474/1‑90 (MQ=255)                      ttATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTa                                                  <  2:556929/90‑1 (MQ=255)                          gCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                              <  2:411912/90‑1 (MQ=255)                            tgtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCt                                            >  1:68738/1‑90 (MQ=255)                             gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtt                                           >  1:311142/1‑90 (MQ=255)                               gAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGaa                                                                                 <  1:242525/50‑1 (MQ=255)                               gAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGaa                                                                                 >  2:242525/1‑50 (MQ=255)                                    aacaaTTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTtat                                    <  2:469245/90‑1 (MQ=255)                                      caaTTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAg                                  <  1:335507/90‑1 (MQ=255)                                             gcCCCCAATAATATATACATGATTGCCAACCATGaa                                                                                 >  1:87468/1‑36 (MQ=38)                                             gcCCCCAATAATATATACATGATTGCCAACCATGaa                                                                                 <  2:87468/36‑1 (MQ=38)                                                cccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATg                        <  1:114804/90‑1 (MQ=255)                                                cccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATg                        >  2:500524/1‑90 (MQ=255)                                                cccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGCGACAGTAAGCCCTTTTTTTATAGTGAAAAGATg                        <  1:202783/90‑1 (MQ=255)                                                          tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt              >  2:340624/1‑90 (MQ=255)                                                            taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc            >  2:244250/1‑90 (MQ=255)                                                            taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc            >  2:411193/1‑90 (MQ=255)                                                            taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc            >  2:354212/1‑90 (MQ=255)                                                            taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc            >  1:444824/1‑90 (MQ=255)                                                            taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGAGGCATCTTACTTtc            >  2:538915/1‑90 (MQ=255)                                                              cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat          >  1:228848/1‑90 (MQ=255)                                                                tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca        <  1:202897/90‑1 (MQ=255)                                                                tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca        >  1:224308/1‑90 (MQ=255)                                                                tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca        >  2:407007/1‑90 (MQ=255)                                                                 gATTGCCAACCATGAAATCAAAAACCTGTTGTTga                                                              >  1:112271/1‑35 (MQ=38)                                                                 gATTGCCAACCATGAAATCAAAAACCTGTTGTTga                                                              <  2:112271/35‑1 (MQ=38)                                                                 gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                              >  2:188026/1‑51 (MQ=255)                                                                 gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                              <  1:188026/51‑1 (MQ=255)                                                                 gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaa       >  1:49925/1‑90 (MQ=255)                                                                 gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaa       >  1:519098/1‑90 (MQ=255)                                                                  aTTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaaa      >  2:192198/1‑90 (MQ=255)                                                                   ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt     >  1:54701/1‑90 (MQ=255)                                                                   ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt     >  2:426285/1‑90 (MQ=255)                                                                     gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATgg   >  1:550184/1‑90 (MQ=255)                                                                      ccAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATggg  >  1:8191/1‑90 (MQ=255)                                                                          |                                                                                      AATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGG  >  NZ_CP009273/3786394‑3786552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑