Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,901,121	A→G	D202D (GAT→GAC)	pstB ←	phosphate ABC transporter ATP‑binding protein PstB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,901,121	0	A	G	100.0%	102.1 / NA	31	D202D (GAT→GAC)	pstB	phosphate ABC transporter ATP‑binding protein PstB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/16);  total (15/16)

ATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAG  >  NZ_CP009273/3901035‑3901198                                                                                       |                                                                              atcaatTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTcc                                                                            >  2:387765/1‑90 (MQ=255)       tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                      <  2:187250/90‑1 (MQ=255)         gCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAg                                                                    >  1:115516/1‑90 (MQ=255)            cAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTc                                                                 <  1:347014/90‑1 (MQ=255)             aGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCg                                                                >  1:198669/1‑90 (MQ=255)                 aCATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGa                                                            <  2:203957/90‑1 (MQ=255)                   aTAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATc                                                          <  2:218798/90‑1 (MQ=255)                        cGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctc                                                     >  1:574062/1‑90 (MQ=255)                            gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                                 >  2:478103/1‑90 (MQ=255)                            gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                                 >  2:341525/1‑90 (MQ=255)                               tgGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATa                                              <  2:72362/90‑1 (MQ=255)                                ggTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATAc                                             >  2:248306/1‑90 (MQ=255)                                   cGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGcc                                          <  2:220479/90‑1 (MQ=255)                                    ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                         >  1:125797/1‑90 (MQ=255)                                    ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                         <  2:577081/90‑1 (MQ=255)                                    ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                         <  2:574062/90‑1 (MQ=255)                                     gAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCg                                        <  1:136370/90‑1 (MQ=255)                                        caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                                     <  1:69934/90‑1 (MQ=255)                                        caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                                     <  1:564890/90‑1 (MQ=255)                                        caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                                     <  2:422113/90‑1 (MQ=255)                                               cAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAg                              >  1:380304/1‑90 (MQ=255)                                                              tgtgGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAac               <  2:190332/90‑1 (MQ=255)                                                               gtgGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAaca              >  2:27664/1‑90 (MQ=255)                                                               gtgGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAaca              >  2:34044/1‑90 (MQ=255)                                                                     gACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtc        <  1:478103/90‑1 (MQ=255)                                                                      aCGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtcg       >  2:1315/1‑90 (MQ=255)                                                                         aTCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                                     <  2:465296/57‑1 (MQ=255)                                                                         aTCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                                     >  1:465296/1‑57 (MQ=255)                                                                           caccacGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGag  >  2:198942/1‑90 (MQ=255)                                                                                  gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACg                                            >  2:391364/1‑41 (MQ=255)                                                                                  gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACg                                            <  1:391364/41‑1 (MQ=255)                                                                                       |                                                                              ATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAG  >  NZ_CP009273/3901035‑3901198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑