Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,064,564	A→G	Y185H (TAT→CAT)	yihS ←	sulfoquinovose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,064,564	0	A	G	100.0%	20.3 / NA	8	Y185H (TAT→CAT)	yihS	sulfoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/4);  total (4/4)

AATGATTATTTCTGGCGACGTCGTGGATAATCACGGAAGCCACGCGAATCGCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATAAACAATCAAGAAAGCTTCCACCGCGTGCATATTGGCATTGCCGCCGCGGT  >  NZ_CP009273/4064477‑4064614                                                                                        |                                                   aaTGATTATTTCTGGCGACGTCGTGGATAATCACGGAAGCCACGCGAATCGCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATgaa                                                  <  1:405157/90‑1 (MQ=255)   tGATTATTTCTGGCGACGTCGTGGATAATCACGGAAGCCACGCGAATCGCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATGAACa                                                <  2:404249/90‑1 (MQ=255)       tattTCTGGCGACGTCGTGGATAATCACGGAAGCCACGCGAATCGCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATGAACAATCa                                            >  1:360371/1‑90 (MQ=255)         tttCTGGCGACGTCGTGGATAATCACGGAAGCCACGCGAATCGCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATGAACAATCAAg                                          >  1:84271/1‑90 (MQ=255)                       gtGGATAATCACGGAAGCCACGCGAATCCCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATGAACAATCAAGAAAGCTTCCACcgc                            >  1:81124/1‑90 (MQ=255)                          gATAATCACGGAAGCCACGCGAATCGCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATGAACAATCAAGAAAGCTTCCACCGCGTg                         <  1:541472/90‑1 (MQ=255)                          gATAATCACGGAAGCCACGCGAATCGCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATGAACAATCAAGAAAGCTTCCACCGCGTg                         <  2:543887/90‑1 (MQ=255)                            tAATCACGGAAGCCACGCGAATCGCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATGAACAATCAAGAAAGCTTCCACCGCGTGCa                       >  2:457527/1‑90 (MQ=255)                                                 tCGCGCGATCCAGCCATTTTTTTTCGTGAGTGACGTCATGAACAATCAAGAAAGCTTCCACCGCGTGCATATTGGCATTGCCGCCGCGGt  <  2:81986/90‑1 (MQ=255)                                                                                        |                                                   AATGATTATTTCTGGCGACGTCGTGGATAATCACGGAAGCCACGCGAATCGCGCGATCCAGCCATTTTTTGTCGTGAGTGACGTCATAAACAATCAAGAAAGCTTCCACCGCGTGCATATTGGCATTGCCGCCGCGGT  >  NZ_CP009273/4064477‑4064614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑