Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	818,875	A→G	N107N (AAT→AAC)	clsB ←	cardiolipin synthase ClsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	818,875	0	A	G	100.0%	24.0 / NA	9	N107N (AAT→AAC)	clsB	cardiolipin synthase ClsB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/4);  total (5/4)

GCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTC  >  NZ_CP009273/818811‑818958                                                                 |                                                                                    gCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAg                                                            >  2:532534/1‑90 (MQ=255)                cTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCgtagt                                             <  2:517832/90‑1 (MQ=255)                       cgcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGaa                                      >  1:413426/1‑90 (MQ=255)                        gcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac                                     >  1:579995/1‑90 (MQ=255)                        gcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac                                     >  2:450991/1‑90 (MQ=255)                         cgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAaca                                    >  1:277229/1‑90 (MQ=255)                                     caATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTg                        <  1:236602/90‑1 (MQ=255)                                          ttGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTc                   <  2:413426/90‑1 (MQ=255)                                                           aaaCACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTc  <  2:134354/90‑1 (MQ=255)                                                                 |                                                                                    GCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTC  >  NZ_CP009273/818811‑818958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑