Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	991,969	T→G	L144F (TTA→TTC)	ssuA ←	aliphatic sulfonate ABC transporter substrate‑binding protein SsuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	991,969	0	T	G	100.0%	27.9 / NA	10	L144F (TTA→TTC)	ssuA	aliphatic sulfonate ABC transporter substrate‑binding protein SsuA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/5);  total (5/5)

TGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTT  >  NZ_CP009273/991925‑992050                                             |                                                                                  tGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGa                                      <  2:278310/90‑1 (MQ=255) tGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGa                                      <  2:70092/90‑1 (MQ=255)            aaaCTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATc                           >  1:242597/1‑90 (MQ=255)            aaaCTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATc                           >  2:498635/1‑90 (MQ=255)                  aaGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTAc                     <  2:206033/90‑1 (MQ=255)                        ggCCTGACGCAGTGCACGCAGGAAAAGGTtgtgtg                                                                      >  1:118470/1‑35 (MQ=38)                        ggCCTGACGCAGTGCACGCAGGAAAAGGTtgtgtg                                                                      <  2:118470/35‑1 (MQ=38)                                gCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGc       <  1:102017/90‑1 (MQ=255)                                     gCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGttt  >  2:318281/1‑90 (MQ=255)                                     gCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGttt  >  2:552168/1‑90 (MQ=255)                                             |                                                                                  TGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTT  >  NZ_CP009273/991925‑992050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑