Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,054,234	A→G	E232G (GAA→GGA)	torC →	pentaheme c‑type cytochrome TorC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,054,234	0	A	G	100.0%	36.1 / NA	13	E232G (GAA→GGA)	torC	pentaheme c‑type cytochrome TorC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/3);  total (10/3)

CGCTGTACTCTATTGATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGAAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGTCAGCG  >  NZ_CP009273/1054153‑1054312                                                                                  |                                                                               cGCTGTACTCTATTGATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGTGAAAg                                                                        <  1:145971/90‑1 (MQ=255)  gCTGTACTCTATTGATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGAGAAAGt                                                                       >  2:236263/1‑90 (MQ=255)               gATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGTGAAAGTCCTTAAACGTGAc                                                          >  1:533157/1‑90 (MQ=255)               gATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGGGAAAGTCCTTAAACGTGAc                                                          >  2:278569/1‑90 (MQ=255)                    tAAGCCAATTTATGCGGCGAGAGGCGATAAAGAAGCCGCTGGTGCTCTGCTGCCTGCTTCGGGAGGGAAAGTCCTTAAACGTGACGGCGa                                                     >  2:524707/1‑90 (MQ=255)                    tAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGTGAAAGTCCTTAAACGTGACGGCGa                                                     >  2:280853/1‑90 (MQ=255)                           aTTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCCGCCTGCTTCGGGAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTg                                              <  1:102300/90‑1 (MQ=255)                                                          ctGGTTCTCTGCTGCCTGCTTCGGGAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAgcg               >  2:407068/1‑90 (MQ=255)                                                                    gctgcCTGCTTCGGGAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCGAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGTCa     >  1:420185/1‑90 (MQ=255)                                                                       gcctgcTTCGGGAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCg            >  1:133808/1‑80 (MQ=255)                                                                       gcctgcTTCGGGAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCg            <  2:133808/80‑1 (MQ=255)                                                                       gcctgcTTCGGGAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGTCAGCg  >  1:250774/1‑90 (MQ=255)                                                                       gcctgcTTCGGGAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGTCAGCg  >  2:202124/1‑90 (MQ=255)                                                                                  |                                                                               CGCTGTACTCTATTGATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGAAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGTCAGCG  >  NZ_CP009273/1054153‑1054312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑