Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,325,886	T→C	G194G (GGT→GGC)	topA →	type I DNA topoisomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,325,886	0	T	C	100.0%	28.1 / NA	10	G194G (GGT→GGC)	topA	type I DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/4);  total (6/4)

CGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAG  >  NZ_CP009273/1325804‑1325956                                                                                   |                                                                       cgcgTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGc                                                                 <  1:557382/90‑1 (MQ=255)                aCCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTc                                                  <  1:252738/90‑1 (MQ=255)                                  tGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtg                                >  1:227003/1‑90 (MQ=255)                                    gTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCt                                              >  1:260342/1‑74 (MQ=255)                                    gTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCt                                              <  2:260342/74‑1 (MQ=255)                                    gTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtgag                              >  2:107786/1‑90 (MQ=255)                                    gTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtgag                              >  2:65961/1‑90 (MQ=255)                                    gTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGGGGTCgagggggag                              >  1:462816/1‑90 (MQ=255)                                                            aTCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCg      >  1:478167/1‑90 (MQ=255)                                                                cTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAag  <  2:443333/90‑1 (MQ=255)                                                                                   |                                                                       CGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAG  >  NZ_CP009273/1325804‑1325956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑