Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,499,566	A→G	S258P (TCC→CCC)	ydcO ←	BenE family transporter YdcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,499,566	0	A	G	85.7%	10.0 / ‑2.9	7	S258P (TCC→CCC)	ydcO	BenE family transporter YdcO
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (5/1);  total (5/2)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

TATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGAAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGA  >  NZ_CP009273/1499488‑1499646                                                                               |                                                                                 tATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTg                                                                       >  2:350282/1‑90 (MQ=255)             cTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGAAAAAACCAGTGCCAGCAATCCAg                                                           <  1:72005/90‑1 (MQ=255)                   ggCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATa                                                     <  2:149934/90‑1 (MQ=255)                       ttGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAAt                                                 >  2:235488/1‑90 (MQ=255)                                                   aTACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCa                     >  1:34687/1‑90 (MQ=255)                                                   aTACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCa                     >  2:205996/1‑90 (MQ=255)                                                                      cGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGa  >  1:505642/1‑90 (MQ=255)                                                                               |                                                                                 TATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGAAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGA  >  NZ_CP009273/1499488‑1499646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑