Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,505,430 A→G K391E (AAA→GAA)  ydcR → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,505,4300AG91.7% 23.9 / ‑3.6 12K391E (AAA→GAA) ydcRPLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (5/6);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

GCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGC  >  NZ_CP009273/1505356‑1505509
                                                                          |                                                                               
gCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATg                                                                  <  2:230926/90‑1 (MQ=255)
 ccTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGa                                                                 >  1:294519/1‑90 (MQ=255)
   tGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACa                                                               <  1:93785/90‑1 (MQ=255)
    gCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAg                                                              <  2:373480/90‑1 (MQ=255)
                      aCGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGtt                                            <  2:461629/90‑1 (MQ=255)
                                cGTGCCTGGCAGGCACTGCTGCGTTATCTGCCGGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTcccc                                  >  2:154662/1‑90 (MQ=255)
                                                 gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg                 <  2:192270/90‑1 (MQ=255)
                                                 gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg                 >  2:299184/1‑90 (MQ=255)
                                                  ctgcTTTATCGGCCTGCGGAGGGGGAAATTCATCAAAATGACAGTGGTTACTTCCTCTGTTTGGAGCCCCCCGAGCCGTTAGATGCCGGc                <  1:13980/90‑1 (MQ=255)
                                                           ctgcctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGc       >  1:292408/1‑90 (MQ=255)
                                                              cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg    >  2:480082/1‑90 (MQ=255)
                                                                tgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggc  <  2:17365/90‑1 (MQ=255)
                                                                          |                                                                               
GCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGC  >  NZ_CP009273/1505356‑1505509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑