| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 115,628 | A→C | T137P (ACG→CCG) | ampD → | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,628 | 0 | A | C | 90.3% | 68.1 / ‑0.0 | 32 | T137P (ACG→CCG) | ampD | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base C (20/8); total (22/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01 | |||||||||||
GCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCC > NZ_CP009273/115542‑115714 | gCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCcgc < 2:481028/90‑1 (MQ=255) aaTGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCgg < 2:341332/90‑1 (MQ=255) aTTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCAc < 1:118257/90‑1 (MQ=255) aTTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCAc < 1:106461/90‑1 (MQ=255) aTTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCAc < 2:161334/90‑1 (MQ=255) ttttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtg > 2:39865/1‑90 (MQ=255) gCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGGTCCGCGGGGACTGATTGATTGCTATCCGGa > 1:84593/1‑90 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTTCTATCCCGGTATCGCTaa > 2:368754/1‑90 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTaa > 2:476804/1‑90 (MQ=255) gATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAc > 2:447937/1‑90 (MQ=255) cGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGa < 1:447937/90‑1 (MQ=255) cTGGCGTATACGGATGCGCAGTATCAACAGCTTGCGGCGGTTAcgc < 2:198085/46‑1 (MQ=255) cTGGCGTATACGGATGCGCAGTATCAACAGCTTGCGGCGGTTAcgc > 1:198085/1‑46 (MQ=255) cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGGTATCGCTAAAAACATGACg > 1:32216/1‑90 (MQ=255) gTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATACCGCTAAAAACATGACGGGCCa > 2:72624/1‑90 (MQ=255) tataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt < 1:476804/90‑1 (MQ=255) ataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCCCGGGCACTTATTGATTGGTATCCGGGTATCGCTAAAAAAATGGCGGGCCGtt > 1:400466/1‑90 (MQ=255) taCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATtg > 2:441581/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGGTATCGCTAAAAAAATGACGGGGCCTTGTGat > 1:48014/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGc > 1:529465/1‑81 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGc < 2:529465/81‑1 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGGCATTGTGat > 1:136385/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGat > 2:252451/1‑90 (MQ=255) gcgcAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGGTTGCTTTCCGGATATCGCTAAAAAAATTACGGGGCCTTGTGATAtt > 1:496183/1‑90 (MQ=255) gcgcAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATc < 2:225158/60‑1 (MQ=255) gcgcAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATc > 1:225158/1‑60 (MQ=255) gcgcAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGGGATAtt > 2:457588/1‑90 (MQ=255) aaCAGCTTGCGGCGGTTTCGCGGGCCCCGGTTGATTGCTATTCGGGTATCGCTAAAAAAATGACGGGCCCTTGTGATATTGCGCCGGGTc > 1:519452/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa > 2:243449/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa > 2:460182/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa > 2:105957/1‑90 (MQ=255) gTTCCGCGGGCACTGATTGATTTCTATCCGGGTATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGGTCGGGAAAACCATccc > 2:349512/1‑90 (MQ=255) | GCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCC > NZ_CP009273/115542‑115714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |