Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,568,198 A→G F417S (TTC→TCC)  pqqL ← insulinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,568,1980AG91.7% 26.1 / ‑3.0 12F417S (TTC→TCC) pqqLinsulinase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (4/7);  total (4/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

TGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAG  >  NZ_CP009273/1568117‑1568261
                                                                                 |                                                               
tgcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGta                                                         <  1:183582/90‑1 (MQ=255)
 gcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGtat                                                        <  2:224661/90‑1 (MQ=255)
                            tAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGAc                             <  2:139516/90‑1 (MQ=255)
                                 tgctgcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTc                        <  1:57409/90‑1 (MQ=255)
                                     gcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGGCTGGTCAGCa                    >  2:297794/1‑90 (MQ=255)
                                     gcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCa                    <  1:234571/90‑1 (MQ=255)
                                      ccACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCAt                   <  2:542318/90‑1 (MQ=255)
                                         caGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACg                <  1:37606/90‑1 (MQ=255)
                                                ttCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCa         >  2:313763/1‑90 (MQ=255)
                                                       gTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGcc                                    <  1:325393/56‑1 (MQ=255)
                                                       gTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGcc                                    >  2:325393/1‑56 (MQ=255)
                                                       gTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAg  >  2:537729/1‑90 (MQ=255)
                                                                                 |                                                               
TGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAG  >  NZ_CP009273/1568117‑1568261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑