Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,575,825	A→T	D319E (GAT→GAA)	ydeN ←	sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,575,825	0	A	T	96.2%	89.0 / ‑4.3	26	D319E (GAT→GAA)	ydeN	sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (15/10);  minor base C (1/0);  total (16/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

GCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCC  >  NZ_CP009273/1575739‑1575900                                                                                       |                                                                            gCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCa                                                                          >  2:301641/1‑90 (MQ=255) gCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCa                                                                          >  2:426938/1‑90 (MQ=255) gCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCa                                                                          >  1:350389/1‑90 (MQ=255) gCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCa                                                                          >  1:512743/1‑90 (MQ=255)  cACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGCTCaa                                                                         >  2:456322/1‑90 (MQ=255)   aCCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAAc                                                                        <  2:512743/90‑1 (MQ=255)    ccgttATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACa                                                                       <  2:432041/87‑1 (MQ=255)              aGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACgg                                                             >  1:203820/1‑90 (MQ=255)              aGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACgg                                                             >  1:80330/1‑90 (MQ=255)               ggTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGa                                                            <  1:114658/90‑1 (MQ=255)                 tAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAg                                                          <  2:401968/90‑1 (MQ=255)                            attgtattgtCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTa                                               >  1:413841/1‑90 (MQ=255)                                    gtCATACTGTCCGGTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGt                                       >  1:545912/1‑90 (MQ=255)                                      cATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGa                                                            <  1:563765/67‑1 (MQ=255)                                      cATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGa                                                            >  2:563765/1‑67 (MQ=255)                                            gTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                               >  2:520447/1‑90 (MQ=255)                                            gTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                               >  2:575227/1‑90 (MQ=255)                                            gTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                               >  1:384528/1‑90 (MQ=255)                                            gTCCGTTTCTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                               >  2:139298/1‑90 (MQ=255)                                             tCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                              <  1:99172/90‑1 (MQ=255)                                              ccGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCg                             >  1:103413/1‑90 (MQ=255)                                                 tttttCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTa                          >  1:447328/1‑90 (MQ=255)                                                                        gCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATc   <  1:426938/90‑1 (MQ=255)                                                                         cGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATcc  <  2:447328/90‑1 (MQ=255)                                                                         cGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATcc  <  2:475071/90‑1 (MQ=255)                                                                         cGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATcc  <  1:300747/90‑1 (MQ=255)                                                                                       |                                                                            GCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCC  >  NZ_CP009273/1575739‑1575900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑