Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,576,388	A→G	S132P (TCC→CCC)	ydeN ←	sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,576,388	0	A	G	100.0%	27.5 / NA	10	S132P (TCC→CCC)	ydeN	sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/3);  total (7/3)

TCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGAT  >  NZ_CP009273/1576309‑1576461                                                                                |                                                                          tCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCgtgt                                                                 <  1:345579/90‑1 (MQ=255)     gAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCca                                                             >  1:511690/1‑90 (MQ=255)     gAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCca                                                             >  2:509989/1‑90 (MQ=255)          tCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAg                                                        >  2:101327/1‑90 (MQ=255)              tATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGt                                                    <  1:149311/90‑1 (MQ=255)                       aGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAAc                                           >  2:174120/1‑90 (MQ=255)                            ccAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACg                                      >  2:577970/1‑90 (MQ=255)                                                    gTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTaa                                              >  1:545154/1‑58 (MQ=255)                                                    gTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTaa                                              <  2:545154/58‑1 (MQ=255)                                                                gcgcGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGAt  >  1:330798/1‑90 (MQ=255)                                                                                |                                                                          TCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGAT  >  NZ_CP009273/1576309‑1576461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑