| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 139,618 | A→G | E118G (GAA→GGA) | yadG → | ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 139,618 | 0 | A | G | 92.3% | 67.3 / ‑0.6 | 26 | E118G (GAA→GGA) | yadG | ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (14/10); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.94e-01 | |||||||||||
TCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAA > NZ_CP009273/139531‑139701 | tCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGgaa > 2:282986/1‑90 (MQ=255) cAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGgaaa > 1:139131/1‑90 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGAACTACGGCGGGGAGCGCAAAGAAGCGGACATCCGCAGCGGAAAg > 2:505020/1‑90 (MQ=255) gTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCt > 1:283992/1‑90 (MQ=255) cGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACt < 2:199768/90‑1 (MQ=255) gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc > 1:559350/1‑90 (MQ=255) gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc > 1:389464/1‑90 (MQ=255) tGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCg < 1:53155/90‑1 (MQ=255) aggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg < 1:135009/53‑1 (MQ=255) aggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg > 2:135009/1‑53 (MQ=255) actacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgcgc < 1:59115/90‑1 (MQ=255) ctacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGcgcg < 1:264714/90‑1 (MQ=255) acGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTa < 1:538745/90‑1 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 1:35414/1‑90 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 1:242210/1‑90 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 1:56083/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 1:104330/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 1:496721/1‑90 (MQ=255) gAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCt > 2:348449/1‑90 (MQ=255) gcgcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTgg > 1:362865/1‑90 (MQ=255) gCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAg > 2:280166/1‑90 (MQ=255) gTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcg > 1:104358/1‑90 (MQ=255) tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc < 2:303286/90‑1 (MQ=255) tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc < 2:486627/90‑1 (MQ=255) ccGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGttt < 2:559350/90‑1 (MQ=255) cGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTa < 2:104330/90‑1 (MQ=255) gCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTaa > 1:251751/1‑90 (MQ=255) gCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGATATCTGGCGGGATGAAGCGCCGTTTaa > 2:252932/1‑90 (MQ=255) | TCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAA > NZ_CP009273/139531‑139701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |