Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,673,542	A→G	K287E (AAG→GAG)	ydgH →	DUF1471 family protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,673,542	0	A	G	100.0%	43.0 / NA	16	K287E (AAG→GAG)	ydgH	DUF1471 family protein YdgH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/11);  total (5/11)

GATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTG  >  NZ_CP009273/1673457‑1673619                                                                                      |                                                                              gatgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCggagaa                                                                           <  2:276073/90‑1 (MQ=255)  atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                          <  1:132835/90‑1 (MQ=255)  atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                          <  1:42757/90‑1 (MQ=255)    ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAgg                                                                        >  2:142093/1‑90 (MQ=255)       cccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGc                                                                     <  1:142093/90‑1 (MQ=255)       cccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGc                                                                     <  2:499427/90‑1 (MQ=255)         cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa                                                                   <  1:461716/90‑1 (MQ=255)         cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa                                                                   <  2:231224/90‑1 (MQ=255)          gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                  <  1:328496/90‑1 (MQ=255)              gACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGCCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAt                                                              >  1:35997/1‑90 (MQ=255)               aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt                                                             <  1:533867/90‑1 (MQ=255)                            ctctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCg                                                >  1:148669/1‑90 (MQ=255)                                                                  aGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTGTTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcg          >  2:144870/1‑90 (MQ=255)                                                                  aGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcg          >  2:221591/1‑90 (MQ=255)                                                                   ggTTGCAACCCGGGCCGCGGAAAAAGGTGCTAAGTTTTACCCCATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc         <  1:64186/90‑1 (MQ=255)                                                                        cGAAACGTGCCGCGGAGAAAGGTGCTAATTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc    <  2:129547/90‑1 (MQ=255)                                                                          aaaCGTGCCGGGGAAAAAGGTGCAAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTg  <  2:265053/90‑1 (MQ=255)                                                                                      |                                                                              GATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTG  >  NZ_CP009273/1673457‑1673619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑