Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	264,689	(A)5→4	intergenic (‑18/+311)	ykfA ← / ← perR	GTPase family protein/LysR substrate‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	264,685	0	A	.	100.0%	54.9 / NA	14	intergenic (‑14/+315)	ykfA/perR	GTPase family protein/LysR substrate‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (6/8);  total (6/8)

CATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAT  >  NZ_CP009273/264613‑264764                                                                         |                                                                                cATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGt                                                               >  1:524246/1‑90 (MQ=255) cATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGt                                                               >  1:656006/1‑90 (MQ=255)   ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg                                                             >  1:123372/1‑90 (MQ=255)   ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg                                                             >  1:517996/1‑90 (MQ=255)      cGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAAt                                                          <  2:265364/90‑1 (MQ=255)                               aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt                                 <  1:231979/90‑1 (MQ=255)                               aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt                                 <  1:551925/90‑1 (MQ=255)                                     ttCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTc                           <  1:310605/90‑1 (MQ=255)                                     ttCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTc                           <  2:444103/90‑1 (MQ=255)                                             ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                   <  1:163269/90‑1 (MQ=255)                                             ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                   <  2:5147/90‑1 (MQ=255)                                              tCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgatg                  <  1:41477/90‑1 (MQ=255)                                                           tcaCTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGaa     >  1:5493/1‑90 (MQ=255)                                                              ctactCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAt  >  1:659408/1‑90 (MQ=255)                                                                         |                                                                                CATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAT  >  NZ_CP009273/264613‑264764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑