| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 172,755 | 0 | A | G | 64.0% | 3.4 / 20.7 | 25 | T388A (ACG→GCG) | clcA | H(+)/Cl(‑) exchange transporter ClcA |
| Reads supporting (aligned to +/- strand): ref base A (1/8); new base G (16/0); total (17/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.32e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGAC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGAT > NZ_CP009273/172666‑172844 | ccccGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGa < 2:121980/90‑1 (MQ=255) cGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGC‑Gt > 2:127615/1‑90 (MQ=255) gggTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGAC‑GTTTGCTATTGCCgg < 1:113118/90‑1 (MQ=255) gtgCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTTGTTTTTCCCGGAAgggg > 1:314690/1‑90 (MQ=255) cTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGC‑GTTTGCTATTGCCGGGATgggggga > 2:35886/1‑88 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGG‑GGTTTTTATTGCCGGGATgggggggtt > 1:403903/1‑86 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGC‑GTTTGCTATTTCCGGAATGGGGGGAtt > 2:381663/1‑90 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGC‑GGTTGCTTTTGCCGGAATGGGGGCAtt > 1:646990/1‑90 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGG‑GGTTTCTTTTTCCGGGATGGGGGGATTATTgg > 1:564283/1‑90 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGC‑GTTTTCTATTTCCGGGATGGGGGGATTACttg > 2:243964/1‑88 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGC‑GTTTGCTATTTCCGGGATGGGGGGATTACttg > 2:485874/1‑88 (MQ=255) gCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGG‑GTTTTCTTTTTTCGGGAGGGGGGGCTTACTggcg > 1:624626/1‑90 (MQ=255) ttCGGAATGGTTGCCGATGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGAC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCa < 2:709613/90‑1 (MQ=255) cGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGG‑GGTTGCTATTTCCGGGATGGGGGGATTACTtgcgggctc > 1:631678/1‑86 (MQ=255) gCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGAC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTcgcgcg < 2:677578/90‑1 (MQ=255) ttGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGAC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGt < 1:241893/90‑1 (MQ=255) gAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGC‑GTTTTCTATTTCCGGGATGGGGGCATTACTGGCGGCATCTATTTGCGCGCCGGTa > 2:570837/1‑90 (MQ=255) cTGTTTCCGCAATATCACCTTGAGGCGGGGAC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACg < 2:220155/90‑1 (MQ=255) ttCCGCAATATCACCTTGAGGCGGGGGC‑GTTTTCTATTTCCGGGATGGGGGGATTTCTGGCGGCATCTATTCGCGCGCCCTTAAAggggg > 2:324534/1‑89 (MQ=255) atatCACCTTGAGGCGGGGAC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCAttct < 2:294148/90‑1 (MQ=255) atatCACCTTGAGGCGGGGAC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCAttct < 2:621716/90‑1 (MQ=255) tatCACCTTGAGGCGGGGGC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTTGCGCGCCGTTAACGGGGGTCAttttg > 2:690679/1‑90 (MQ=255) aCCTTGAGGCGGGGGC‑GTTTTCTATTTCCGGGATGGGGGGATTACTGGCGGGATCTATTTGCGCGCCGTTTACGGGGGTCAtttttgttt > 1:294148/1‑89 (MQ=255) aCCTTGAGGCGGGGGC‑GTTTTCTATTGCCGGGATGGGGGGATTACTGGCGGCATCTATTTGCGCGCCCTTTACGGGGGTCAttttggttc > 1:138510/1‑90 (MQ=255) aCCTTGAGGCGGGGAC‑GTTTTCTATTTCCGGAATGGGGGCATTACTGGCGGCATCTATTCTCGCGCCCTTAAAGGGGGTCAttctgggtc > 2:321791/1‑90 (MQ=255) gggAC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGAcc < 1:573652/90‑1 (MQ=255) aC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGAt < 1:37662/90‑1 (MQ=255) | CCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGAC‑GTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGAT > NZ_CP009273/172666‑172844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |