| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 721,800 | 0 | G | A | 57.9% | ‑2.4 / 15.4 | 19 | V231V (GTC→GTT) | kdpB | potassium‑transporting ATPase subunit KdpB |
| Reads supporting (aligned to +/- strand): ref base G (1/7); new base A (11/0); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCC > NZ_CP009273/721713‑721888 | gacCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAAAc > 2:681237/1‑90 (MQ=255) gcagcGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAAACGAAAg > 1:54911/1‑90 (MQ=255) cgcCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAg < 1:685249/90‑1 (MQ=255) gcCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAgg > 2:488126/1‑90 (MQ=255) ccaccaGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAggg < 1:218307/90‑1 (MQ=255) cGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAAACGATAGTCAGGGCAATcagcag > 2:415006/1‑90 (MQ=255) tACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAAACGATAGGCAGGGGAATCAGCCGAAt > 2:536904/1‑90 (MQ=255) gCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCCGTAAAAAAACCAAAGGCAGGGGCATCAACCGGATggg > 1:416510/1‑89 (MQ=255) cTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTc < 1:57902/90‑1 (MQ=255) cTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTc < 1:199525/90‑1 (MQ=255) gCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAAACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATttcgtt > 2:181558/1‑90 (MQ=255) gCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAAACGATAGTCAGGGCAATCAGCAGAATGGGGAGGGCAATttcgtt > 1:106271/1‑90 (MQ=255) gCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAAACGAAAGTCAGGGCAAACAGCAGAAAGGGCAGGGCAATtttgtt > 1:163692/1‑90 (MQ=255) gCCCCACGCGGAAAACGACCACGGCGGGGCGGTTGCCCGAAAAAAAACGAAAGCAAGGGGAATCCGGCAAATGGGGAGGGGAAttttttt > 1:664002/1‑86 (MQ=255) gTGGCGGTTGCCAGTAAAAAAACGATAGTCAGGGCAAACAGCAGAAAGGGCAGGGCAATTTCGTTCGGGGGTTTTCGGCGCTGTGCGccc > 1:304960/1‑89 (MQ=255) gCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTcc < 2:641860/90‑1 (MQ=255) tGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATCTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCAt < 2:139824/90‑1 (MQ=255) tGCCAGTAAAAAAACGATAGTCAGGGCAATCAGCAGAATGGGCAGGGCAATTTCGTTCGGCGGTTTTCGTCGCTGTGCGCCTTCCACCAt > 1:220107/1‑90 (MQ=255) aGACGAAAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATcc < 1:42370/90‑1 (MQ=255) | GACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCC > NZ_CP009273/721713‑721888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |