| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 760,181 | 0 | G | C | 64.3% | 0.0 / 11.6 | 14 | P182P (CCG→CCC) | sucD | succinate‑‑CoA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (0/5); new base C (9/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCG > NZ_CP009273/760104‑760266 | cTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCCGGctct > 2:18943/1‑90 (MQ=255) aTGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCACCCCCGGCTCTTACtttt > 1:624783/1‑89 (MQ=255) gCGGTTAATCAGACCACGGATTACGGTTTCGGTCATTCGACCTGTGTCGGTATCGGCGGTAACCCCCCCCCTGGGTCTACCTTTTTccgc > 2:200204/1‑87 (MQ=255) gACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGACCCCCCCCCCGGCCCCCACTTTTTCTACATTCTCGCAAt > 1:521487/1‑90 (MQ=255) aCCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGGCCCCATCCCCGGCCCTTACTTTTTTGACATTTTCCaaaat > 2:417328/1‑88 (MQ=255) ccACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGACCCCACCCCCGGGGCTAACTTTTTTTACAATCTCTAAAAGt > 1:224982/1‑90 (MQ=255) aCGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCCGGCTCTAACTTTTTTGACATTTTCGAAAAGttt > 2:672260/1‑89 (MQ=255) gcccTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGc < 2:5628/88‑1 (MQ=255) gACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGc < 2:393913/90‑1 (MQ=255) gtCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGtg < 1:382837/90‑1 (MQ=255) gtCGGTATCGGCGGTGACCCCATCCCCGGCTCTAACTTTTTCGACATTTTCGAAATGTTTGAAAAAAATCCGCAGACCGAAGCGATCGtg > 2:560147/1‑90 (MQ=255) ggTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGtgatg < 1:478273/90‑1 (MQ=255) gTGACCCCATCCCCGGCTCTAACTTTTTTGACATTCTCGAAATGTTTGAAAAAAAACCGCCGACCGAAACGAGCGTGGTGGTCGGGgaga > 1:274455/1‑90 (MQ=255) aCCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcg < 1:627085/90‑1 (MQ=255) | CTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCG > NZ_CP009273/760104‑760266 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |