Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,319,9290TG65.4% 2.1 / 25.1 26G114G (GGT→GGG) atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/9);  new base G (17/0);  total (17/9)
Fisher's exact test for biased strand distribution p-value = 3.20e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-05
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGA  >  NZ_CP009273/2319842‑2320012
                                                                                       |                                                                                   
tAAAGTATGTGATTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTAt                                                                                   <  1:48326/90‑1 (MQ=255)
     tATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGaaa                                                                              >  2:353076/1‑90 (MQ=255)
       tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGgaaaa                                                                            >  2:99152/1‑89 (MQ=255)
       tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGgaaaa                                                                            >  2:622817/1‑89 (MQ=255)
       tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGaaaaa                                                                            >  2:494689/1‑89 (MQ=255)
           gTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGa                                                                        >  1:106040/1‑90 (MQ=255)
             tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATTAgg                                                                      >  2:675073/1‑89 (MQ=255)
              cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGtt                                                                     >  2:109020/1‑90 (MQ=255)
                    ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccc                                                               <  1:338306/90‑1 (MQ=255)
                    ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccc                                                               <  1:471725/90‑1 (MQ=255)
                    ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccc                                                               <  1:713521/90‑1 (MQ=255)
                            gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCcactta                                                       >  2:335467/1‑90 (MQ=255)
                               gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTc                                                    <  1:335467/90‑1 (MQ=255)
                                    tGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGAGTTTTGCCCCCCACTTTCTCGATGc                                               >  1:195217/1‑90 (MQ=255)
                                                      ggcaggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                             <  2:15356/90‑1 (MQ=255)
                                                         aggTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGGTATCg                          >  2:287059/1‑90 (MQ=255)
                                                                    agagCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCCACTTTCTCGATGCAAAAACACGCTCTGGGTTTTGTCTTTGGGACg               >  1:20946/1‑90 (MQ=255)
                                                                    agagCATTGTGGCGGGGGGGATGGAAAATATGAGGTTAGCCCCCCACTTACTCGATGCAAAAGCACGCCCTGGGTATCGTCTTTGGGACg               >  2:435298/1‑90 (MQ=255)
                                                                     gagCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCCCCTTACTCGATGCAAAAACACGCTCTGGGTATCGTCTTGGGGACgg              >  1:447062/1‑90 (MQ=255)
                                                                       gCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc            <  2:13619/90‑1 (MQ=255)
                                                                       gCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc            <  1:529463/90‑1 (MQ=255)
                                                                         aTTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc            <  1:584499/88‑1 (MQ=255)
                                                                         aTTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc            >  2:584499/1‑88 (MQ=255)
                                                                                 ggggggGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGAGACGGACAGGGTTATGa  >  2:265733/1‑90 (MQ=255)
                                                                                 ggggggGATGGAAAAAATTAGGTTTGCCCCCCACTTACTCGATTCAAAAACACGCCCTGGGTATCGTCTTGGAGACGGGCAGGgttttta  >  1:723107/1‑86 (MQ=255)
                                                                                 ggggggGATGGAAAAAATGAGGTTAGCCCCCTACTTACTCGATGCAAAAACACCCTCTGGGTATCGTCTTTGGGAAGGGCAGGGTTTTGa  >  2:413223/1‑90 (MQ=255)
                                                                                       |                                                                                   
TAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGA  >  NZ_CP009273/2319842‑2320012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑