| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,336,778 | 0 | T | A | 56.2% | 6.7 / 16.3 | 16 | M291L (ATG→TTG) | yfaL | AIDA‑I family autotransporter adhesin YfaL/EhaC |
| Reads supporting (aligned to +/- strand): ref base T (6/1); new base A (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTC > NZ_CP009273/2336689‑2336857 | gTCAACGGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACAt > 1:20996/1‑90 (MQ=255) atcctctGTATTCCAAAAACCCGGCTTTTTTCCGTCGGAAATATAAAAGGTACCTTCATTTAAGCCGAGATAAAAAAAGCCACCcgccgc < 2:629401/87‑1 (MQ=255) ttCTCTGTATTGCAAATACCCAGCGTTTTTCCGTCGGCAATATCAAGGGAAACTTCACTTAAGCCAAAATAAAAAAAGCCCCCCGCCGCa < 1:587502/90‑1 (MQ=255) ctGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGagg > 2:142600/1‑90 (MQ=255) ccAATACCCAGCTTTTTTCCGTCGGAAATATCAAAGGTAACTTCCCTTAACCCGAGATACAAAAACCCCCCCGCCGCAGAGGAAGGACCa < 2:481275/90‑1 (MQ=255) caaaTACCCAGCTTTTTCCCGTCGGAAATATAAAAGGTAACTTCACTTAAGCCGAGATAAAAAAAGCCCCCCGCCGCAGAGGAAGGACCa < 1:41821/88‑1 (MQ=255) aaaTAACCAGCTTTTTTCCGTCGGCAATATAAAAGGTAACTTCACTTAAGCCGAGATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCAt < 1:62218/89‑1 (MQ=255) tAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCAtctc > 2:49539/1‑90 (MQ=255) tAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCAtctc > 2:652597/1‑90 (MQ=255) cccAGCTTTTTCCCGTCGGCAATATCAAAGGTAACTTCCCTTAAGCCGAGATACATAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCa < 2:69203/89‑1 (MQ=255) aGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAg > 2:405798/1‑90 (MQ=255) tttcCCGTGGGCAATATAAAAGGTACCTTCATTTAAGCGGAGATAAAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCctgc < 1:583913/90‑1 (MQ=255) tCCGTCGGAAATATAAAAGGTAACTCCACTAAAGCCGAGAAACAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCtgctgc < 1:142600/90‑1 (MQ=255) cccgccgGCAATACAAAAGGAACCTCCACTTAAGCCGAAATAAAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCtgctgc < 1:550863/89‑1 (MQ=255) tAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTa > 1:445149/1‑90 (MQ=255) aCTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAac > 2:154992/1‑90 (MQ=255) ccGAAATAAAAAAACCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTc < 2:621394/90‑1 (MQ=255) | GTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTC > NZ_CP009273/2336689‑2336857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |